CPC C12Q 1/6818 (2013.01) [C12Q 1/6804 (2013.01)] | 15 Claims |
1. A method for analyzing a planar sample, the method comprising:
(a) labeling the planar sample with a capture agent to produce a labeled sample,
wherein
the capture agent is linked to a nucleic acid comprising an overhang sequence;
and
(ii) the capture agent comprises an antibody;
(b) contacting the labeled sample with a labeled oligonucleotide, wherein the labeled oligonucleotide comprises an fluorescent dye optical label and hybridizes to at least a portion of the overhang sequence,
(c) contacting the labeled sample with a ligase, thereby ligating the labeled oligonucleotide to the nucleic acid; and
(d) reading a signal generated by the labeled oligonucleotide, wherein the reading comprises fluorescent microscopy.
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