US 12,331,344 B2
On-slide staining by primer extension
Nikolay Samusik, Mountain View, CA (US); Garry P. Nolan, Redwood City, CA (US); Yury Goltsev, Stanford, CA (US); and David Robert McIlwain, Palo Alto, CA (US)
Assigned to THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY, Stanford, CA (US)
Filed by The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Filed on Mar. 2, 2023, as Appl. No. 18/177,750.
Application 18/177,750 is a continuation of application No. 17/185,757, filed on Feb. 25, 2021, granted, now 11,634,753.
Application 17/185,757 is a continuation of application No. 16/679,769, filed on Nov. 11, 2019, granted, now 10,982,263, issued on Apr. 20, 2021.
Application 16/679,769 is a continuation of application No. 15/317,019, abandoned, previously published as PCT/US2015/036763, filed on Jun. 19, 2015.
Application 16/679,769 is a continuation of application No. 14/560,921, filed on Dec. 4, 2014, granted, now 9,909,167, issued on Mar. 6, 2018.
Claims priority of provisional application 62/015,799, filed on Jun. 23, 2014.
Prior Publication US 2024/0035068 A1, Feb. 1, 2024
Int. Cl. C12Q 1/6869 (2018.01); C12Q 1/6804 (2018.01); C12Q 1/6818 (2018.01)
CPC C12Q 1/6818 (2013.01) [C12Q 1/6804 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method for analyzing a planar sample, the method comprising:
(a) labeling the planar sample with a capture agent to produce a labeled sample,
wherein
the capture agent is linked to a nucleic acid comprising an overhang sequence;
and
(ii) the capture agent comprises an antibody;
(b) contacting the labeled sample with a labeled oligonucleotide, wherein the labeled oligonucleotide comprises an fluorescent dye optical label and hybridizes to at least a portion of the overhang sequence,
(c) contacting the labeled sample with a ligase, thereby ligating the labeled oligonucleotide to the nucleic acid; and
(d) reading a signal generated by the labeled oligonucleotide, wherein the reading comprises fluorescent microscopy.