	US 12,331,339 B2
	Method of producing sulfur-containing amino acid or derivative thereof
Sol Choi, Seoul (KR); Hee Ju Kim, Seoul (KR); Jin Ah Rho, Seoul (KR); Jin Nam Lee, Seoul (KR); Han Hyoung Lee, Seoul (KR); Sun Young Lee, Seoul (KR); Sang Jun Kim, Seoul (KR); and Jihyun Shim, Seoul (KR)
Assigned to CJ CHEILJEDANG CORPORATION, Seoul (KR)
Appl. No. 17/597,007
Filed by CJ CHEILJEDANG CORPORATION, Seoul (KR)
PCT Filed Jun. 26, 2020, PCT No. PCT/KR2020/008415 § 371(c)(1), (2) Date Dec. 22, 2021, PCT Pub. No. WO2020/263043, PCT Pub. Date Dec. 30, 2020.
Claims priority of application No. 10-2019-0077999 (KR), filed on Jun. 28, 2019.
Prior Publication US 2022/0315963 A1, Oct. 6, 2022
Int. Cl. C12P 13/12 (2006.01); C07K 14/245 (2006.01); C07K 14/34 (2006.01); C12N 15/70 (2006.01); C12N 15/77 (2006.01)

CPC C12P 13/12 (2013.01) [C07K 14/245 (2013.01); C07K 14/34 (2013.01); C12N 15/70 (2013.01); C12N 15/77 (2013.01)]

7 Claims

1. A method of producing a sulfur-containing amino acid or a derivative of the sulfur-containing amino acid, the method comprising culturing a genetically modified microorganism in a culture medium including thiosulfate, and recovering the sulfur-containing amino acid or the derivative of the sulfur-containing amino acid from the microorganism or the culture medium,

wherein the microorganism comprises a genetic modification to increase an expression level of a protein encoded by ssuD gene compared to a non-modified microorganism,

wherein the production of the sulfur-containing amino acid by the microorganism comprising the genetic modification is increased compared to the non-modified microorganism;

wherein the protein encoded by the ssuD gene comprises an amino acid sequence having at least 80% sequence identity to SEQ ID NO: 43.