US 12,331,337 B2
Fe-S fusion protein acting as electron transfer chain, carbon monoxide formate redox enzyme mediated through FES fusion protein, strain BCF12 derived from Thermococcus wherein enzyme is transformed, and use thereof
Jung-Hyun Lee, Busan (KR); Jae Kyu Lim, Busan (KR); Hyun Sook Lee, Busan (KR); Sung Gyun Kang, Busan (KR); Kae Kyoung Kwon, Busan (KR); Yun Jae Kim, Busan (KR); and Ji-In Yang, Busan (KR)
Assigned to KOREA INSTITUTE OF OCEAN SCIENCE & TECHNOLOGY, Busan (KR)
Appl. No. 17/292,967
Filed by KOREA INSTITUTE OF OCEAN SCIENCE & TECHNOLOGY, Busan (KR)
PCT Filed Nov. 28, 2018, PCT No. PCT/KR2018/014807
§ 371(c)(1), (2) Date May 11, 2021,
PCT Pub. No. WO2020/101100, PCT Pub. Date May 22, 2020.
Claims priority of application No. 10-2018-0138462 (KR), filed on Nov. 12, 2018; application No. 10-2018-0138467 (KR), filed on Nov. 12, 2018; and application No. 10-2018-0138475 (KR), filed on Nov. 12, 2018.
Prior Publication US 2022/0213463 A1, Jul. 7, 2022
Int. Cl. C12N 9/96 (2006.01); C12N 9/02 (2006.01); C12N 15/52 (2006.01); C12P 7/40 (2006.01)
CPC C12N 9/96 (2013.01) [C12N 9/0008 (2013.01); C12N 15/52 (2013.01); C12P 7/40 (2013.01); C12Y 102/01002 (2013.01); C12Y 102/02001 (2013.01); C12Y 102/07004 (2013.01)] 2 Claims
 
1. An Fe—S fusion protein comprising:
a flexible linker having the amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 6; and
two or more Fe—S proteins, each comprising any one amino acid sequence selected from the group consisting of SEQ ID NOs: 7 to 11, the two or more Fe—S proteins covalently linked together through the flexible linker, the Fe—S fusion protein capable of acting as an electron transport chain functioning as a channel through which electrons move,
wherein the Fe—S fusion protein comprises the amino acid sequence of SEQ ID NO: 13.