	US 12,331,325 B2
	Engineered transaminase polypeptides
Scott J. Novick, Palo Alto, CA (US); and Nikki Dellas, San Carlos, CA (US)
Assigned to Codexis, Inc., Redwood City, CA (US)
Filed by Codexis, Inc., Redwood City, CA (US)
Filed on Sep. 1, 2023, as Appl. No. 18/459,797.
Application 18/459,797 is a division of application No. 17/223,677, filed on Apr. 6, 2021, granted, now 11,788,071.
Claims priority of provisional application 63/008,047, filed on Apr. 10, 2020.
Prior Publication US 2024/0002816 A1, Jan. 4, 2024
Int. Cl. C12N 9/10 (2006.01)

CPC C12N 9/1096 (2013.01)

11 Claims

1. An engineered polynucleotide encoding an engineered transaminase having an amino acid sequence of at least 85% identity to SEQ ID NO: 8, or a functional fragment thereof, and a set of substitutions at positions 21/163/323/408, in said amino acid sequence , wherein the positions of said substitutions are numbered with reference to SEQ ID NO: 8 and wherein the engineered transaminase exhibits an increased enzymatic activity relative to an engineered transaminase having the amino acid sequence set forth in SEQ ID NO:8.