US 12,331,324 B2
Versatile peroxidases and uses thereof
Sarel Fleishman, Rehovot (IL); and Shiran Barber-Zucker, Rehovot (IL)
Assigned to Yeda Research and Development Co. Ltd., Rehovot (IL)
Filed by Yeda Research and Development Co. Ltd., Rehovot (IL)
Filed on May 23, 2024, as Appl. No. 18/672,113.
Application 18/672,113 is a continuation of application No. PCT/IL2022/051256, filed on Nov. 24, 2022.
Claims priority of provisional application 63/282,678, filed on Nov. 23, 2021.
Prior Publication US 2024/0301368 A1, Sep. 12, 2024
Int. Cl. C12N 15/18 (2006.01); C12N 9/08 (2006.01); C12N 15/81 (2006.01); C12P 7/10 (2006.01)
CPC C12N 9/0065 (2013.01) [C12N 15/81 (2013.01); C12P 7/10 (2013.01); C12Y 111/01007 (2013.01)] 18 Claims
 
1. A peroxidase enzyme comprising:
(i) an amino acid sequence at least 85% identical to SEQ ID NO: 2; and
(ii) mutations set forth in R4P, M5S, E34T, G59A, T65W, A67Q, V85I, V95I, P104H, I107Q, T125V, S135P, S141P, D143P, S161Q, S164A, S167A, D172S, D229Q, A247L, S249A, S252P, R265Q, A266D, L269Q, A270S, E273A, G274A, A288S, T290I, A299P, T300P, S323D, A325P, S330P, A333P, A335P and A341P, wherein coordinates of said mutations correspond to said SEQ ID NO: 2;
wherein the peroxidase enzyme is characterized by increased yield when expressed in yeast cells as compared to wild-type peroxidase enzyme having an amino acid sequence as set forth in SEQ ID NO: 5.