	US 12,331,322 B2
	Agent for accelerating growth of pluripotent stem cells
Jun Nojima, Tokyo (JP); Hidenori Matsuo, Nagahama (JP); Yuriko Furuya, Nagahama (JP); and Hisataka Yasuda, Nagahama (JP)
Assigned to ORIENTAL YEAST CO., LTD., Tokyo (JP)
Filed by Oriental Yeast Co., Ltd., Tokyo (JP)
Filed on Dec. 12, 2022, as Appl. No. 18/064,441.
Application 18/064,441 is a continuation of application No. 16/481,760, granted, now 11,634,690, previously published as PCT/JP2018/003172, filed on Jan. 31, 2018.
Claims priority of application No. 2017-015313 (JP), filed on Jan. 31, 2017.
Prior Publication US 2023/0115507 A1, Apr. 13, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/074 (2010.01); C12N 5/0775 (2010.01)

CPC C12N 5/0696 (2013.01) [C12N 5/0662 (2013.01); C12N 2501/999 (2013.01)]

7 Claims

1. A method for accelerating growth of a mesenchymal stem cell, comprising:

culturing the mesenchymal stem cell in a maintenance culture medium that comprises a β-nicotinamide mononucleotide, or a pharmaceutically acceptable salt thereof, or a solvate thereof, wherein

a concentration of the β-nicotinamide mononucleotide of the maintenance culture medium is in a range from 0.05 to 2.0 mM, and

mesenchymal stem cell growth occurs while maintaining multipotency thereof.