	US 12,331,316 B2
	T cell which expresses a gamma-delta t cell receptor (TCR) and a chimeric antigen receptor (CAR)
John Anderson, London (GB); Jonathan Fisher, London (GB); Martin Pulé, London (GB); and Kenth Gustafsson, London (GB)
Assigned to UCL Business Ltd, London (GB)
Filed by UCL Business Ltd, London (GB)
Filed on Nov. 17, 2021, as Appl. No. 17/528,836.
Application 17/528,836 is a continuation of application No. 15/567,165, abandoned, previously published as PCT/GB2016/051235, filed on Apr. 29, 2016.
Claims priority of application No. 1507368 (GB), filed on Apr. 30, 2015.
Prior Publication US 2022/0211756 A1, Jul. 7, 2022
Int. Cl. A61K 40/31 (2025.01); A61K 40/11 (2025.01); A61K 40/42 (2025.01); A61P 31/04 (2006.01); A61P 31/12 (2006.01); A61P 35/00 (2006.01); C07K 14/725 (2006.01); C12N 5/0783 (2010.01)

CPC C12N 5/0638 (2013.01) [A61K 40/11 (2025.01); A61K 40/31 (2025.01); A61K 40/4202 (2025.01); A61K 40/4258 (2025.01); A61P 31/04 (2018.01); A61P 31/12 (2018.01); A61P 35/00 (2018.01); C07K 14/7051 (2013.01); C12N 5/0636 (2013.01); A61K 2239/28 (2023.05); A61K 2239/29 (2023.05); C12N 2501/599 (2013.01); C12N 2510/00 (2013.01)]

10 Claims

1. A T cell which expresses a gamma-delta T cell receptor (TCR) and a chimeric antigen receptor (CAR), wherein the TCR is used to provide a signal 1 for T cell activation and the CAR is used to provide a co-stimulatory signal 2, and the CAR comprises the amino acid sequence of SEQ ID NO: 2;

wherein binding of a first antigen to the gamma-delta TCR results in signal 1 production, and

wherein binding of a second antigen to an antigen-binding domain of the CAR results in a co-stimulatory intracellular signaling domain of the CAR transmitting the co-stimulatory signal 2, but not the signal 1, to the T cell,

wherein the T cell is activated and capable of killing a target cell which expresses the first antigen capable of binding to the gamma delta TCR and the second antigen capable of binding to the CAR.