US 12,331,314 B2
Culture method for organoids
Meritxell Huch Ortega, Utrecht (NL); Johannes Carolus Clevers, Utrecht (NL); and Sylvia Fernandez Boj, Utrecht (NL)
Assigned to Koninklijke Nederlandse Akademie Van Wetenschappen, Utrecht (NL)
Filed by Koninklijke Nederlandse Akademie Van Wetenschappen, Utrecht (NL)
Filed on Jun. 19, 2020, as Appl. No. 16/906,515.
Application 16/906,515 is a division of application No. 16/743,191, filed on Jan. 15, 2020.
Application 16/743,191 is a division of application No. 15/310,905, granted, now 10,597,633, issued on Mar. 24, 2020, previously published as PCT/EP2015/060815, filed on May 15, 2015.
Claims priority of application No. 1408764 (GB), filed on May 16, 2014; and application No. 1422184 (GB), filed on Dec. 12, 2014.
Prior Publication US 2020/0318063 A1, Oct. 8, 2020
Int. Cl. C12N 5/071 (2010.01); A61K 35/39 (2015.01); A61K 35/407 (2015.01)
CPC C12N 5/0602 (2013.01) [A61K 35/39 (2013.01); A61K 35/407 (2013.01); C12N 5/067 (2013.01); C12N 5/0671 (2013.01); C12N 5/0676 (2013.01); C12N 5/0677 (2013.01); C12N 2501/01 (2013.01); C12N 2501/11 (2013.01); C12N 2501/115 (2013.01); C12N 2501/119 (2013.01); C12N 2501/12 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/19 (2013.01); C12N 2501/30 (2013.01); C12N 2501/345 (2013.01); C12N 2501/415 (2013.01); C12N 2501/42 (2013.01); C12N 2501/999 (2013.01); C12N 2503/02 (2013.01); Y02A 50/30 (2018.01)] 15 Claims
 
1. A differentiation medium comprising a basal medium for animal or human cells to which is added one or more receptor tyrosine kinase ligands, a Notch inhibitor, a compound that induces the phosphorylation of SMAD1 or SMAD5, and a TGF-beta inhibitor.