US 12,331,313 B2
Tunable silk-based biomaterials with saccharide substitutions, and methods of producing the same
David L. Kaplan, Concord, MA (US); Jugal Kishore Sahoo, Belmont, MA (US); Onur Hasturk, Medford, MA (US); and Jaewon Choi, Cambridge, MA (US)
Assigned to Trustees of Tufts College, Medford, MA (US)
Filed by Trustees of Tufts College, Medford, MA (US)
Filed on Jul. 14, 2023, as Appl. No. 18/352,366.
Application 18/352,366 is a continuation of application No. PCT/US2022/070215, filed on Jan. 17, 2022.
Claims priority of provisional application 63/138,193, filed on Jan. 15, 2021.
Prior Publication US 2024/0132836 A1, Apr. 25, 2024
Int. Cl. C07K 14/435 (2006.01); A61L 27/36 (2006.01); C12N 5/00 (2006.01); A61L 27/52 (2006.01); A61L 27/56 (2006.01)
CPC C12N 5/0068 (2013.01) [A61L 27/3604 (2013.01); C07K 14/43586 (2013.01); A61L 27/52 (2013.01); A61L 27/56 (2013.01); C12N 2533/50 (2013.01); C12N 2537/10 (2013.01)] 18 Claims
 
1. A composition comprising a saccharide-substituted silk fibroin, the saccharide-substituted silk fibroin comprising at least one saccharide covalently coupled to silk fibroin via a serine residue on the silk fibroin, wherein the serine residue is modified by carboxylation, wherein the at least one saccharide has an amine group and is selected from the group consisting of a monosaccharide, a disaccharide, and an oligosaccharide, and wherein a linking agent covalently couples the modified serine residue to the amine group of the at least one saccharide.