US 12,331,311 B2
Protein modification of living cells using sortase
Lee Kim Swee, Heidelberg (DE); and Hidde L. Ploegh, Jamaica Plain, MA (US)
Assigned to Whitehead Institute for Biomedical Research, Cambridge, MA (US)
Filed by Whitehead Institute for Biomedical Research, Cambridge, MA (US)
Filed on Feb. 15, 2019, as Appl. No. 16/277,721.
Application 16/277,721 is a continuation of application No. 14/890,296, granted, now 10,260,038, previously published as PCT/US2014/037545, filed on May 9, 2014.
Claims priority of provisional application 61/943,094, filed on Feb. 21, 2014.
Claims priority of provisional application 61/822,092, filed on May 10, 2013.
Prior Publication US 2019/0256818 A1, Aug. 22, 2019
Int. Cl. C12N 5/00 (2006.01); A61K 40/11 (2025.01); A61K 40/15 (2025.01); A61K 40/31 (2025.01); A61K 40/32 (2025.01); A61K 40/42 (2025.01); A61K 47/65 (2017.01); A61K 47/66 (2017.01); A61K 47/68 (2017.01); C12N 5/0783 (2010.01); C12N 9/52 (2006.01)
CPC C12N 5/0006 (2013.01) [A61K 40/11 (2025.01); A61K 40/15 (2025.01); A61K 40/31 (2025.01); A61K 40/32 (2025.01); A61K 40/4257 (2025.01); A61K 40/4272 (2025.01); A61K 40/428 (2025.01); A61K 47/65 (2017.08); A61K 47/66 (2017.08); A61K 47/68 (2017.08); C12N 5/0636 (2013.01); C12N 5/0638 (2013.01); C12N 9/52 (2013.01); C12Y 304/2207 (2013.01)] 25 Claims
 
1. A human cell having an agent linked thereto via a sortase recognition sequence, wherein the agent is linked to an endogenous, non-genetically engineered protein of the human cell, wherein the non-genetically engineered protein comprises an endogenous nucleophilic acceptor sequence to which the agent is linked via the sortase recognition sequence, wherein the human cell has not been genetically engineered to express a protein comprising a sortase recognition sequence or a nucleophilic acceptor sequence, and wherein the agent comprises a cytokine a costimulatory molecule, or an adjuvant, wherein the adjuvant is a CD40 ligand, a ligand of a TLR, a pathogen-derived molecular pattern (PAMP), a PAMP mimic, an immunostimulatory nucleic acid, or a cationic polymer.