CPC C12N 5/0006 (2013.01) [A61K 40/11 (2025.01); A61K 40/15 (2025.01); A61K 40/31 (2025.01); A61K 40/32 (2025.01); A61K 40/4257 (2025.01); A61K 40/4272 (2025.01); A61K 40/428 (2025.01); A61K 47/65 (2017.08); A61K 47/66 (2017.08); A61K 47/68 (2017.08); C12N 5/0636 (2013.01); C12N 5/0638 (2013.01); C12N 9/52 (2013.01); C12Y 304/2207 (2013.01)] | 25 Claims |
1. A human cell having an agent linked thereto via a sortase recognition sequence, wherein the agent is linked to an endogenous, non-genetically engineered protein of the human cell, wherein the non-genetically engineered protein comprises an endogenous nucleophilic acceptor sequence to which the agent is linked via the sortase recognition sequence, wherein the human cell has not been genetically engineered to express a protein comprising a sortase recognition sequence or a nucleophilic acceptor sequence, and wherein the agent comprises a cytokine a costimulatory molecule, or an adjuvant, wherein the adjuvant is a CD40 ligand, a ligand of a TLR, a pathogen-derived molecular pattern (PAMP), a PAMP mimic, an immunostimulatory nucleic acid, or a cationic polymer.
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