US 12,331,310 B2
Cell membrane penetrating conjugates for gene editing
Nusrat Sanghamitra, Cork (IE)
Assigned to CYGENICA LIMITED, Cork (IE)
Filed by CYGENICA LIMITED, Cork (IE)
Filed on Aug. 18, 2021, as Appl. No. 17/405,863.
Application 17/405,863 is a continuation of application No. PCT/EP2020/055201, filed on Feb. 27, 2020.
Claims priority of application No. 1902648 (GB), filed on Feb. 27, 2019.
Prior Publication US 2022/0056483 A1, Feb. 24, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/10 (2006.01); C12N 9/10 (2006.01); C12N 9/22 (2006.01); C12N 9/88 (2006.01); C12N 15/113 (2010.01); C12N 15/90 (2006.01)
CPC C12N 15/90 (2013.01) [C12N 9/1029 (2013.01); C12N 9/22 (2013.01); C12N 9/88 (2013.01); C12N 15/102 (2013.01); C12N 15/113 (2013.01); C07K 2319/04 (2013.01); C07K 2319/07 (2013.01); C07K 2319/09 (2013.01); C12N 2310/20 (2017.05); C12Y 203/01129 (2013.01); C12Y 402/02 (2013.01)] 24 Claims
OG exemplary drawing
 
1. A genome-editing complex for modifying a target polynucleotide comprising a recombinant β helical protein, represented by a sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID: 2, SEQ ID: 3, SEQ ID: 4, SEQ ID: 5, SEQ ID: 6, SEQ ID: 7, SEQ ID: 8, SEQ ID: 9, SEQ ID: 10, SEQ ID: 11, SEQ ID: 12, and combinations thereof, wherein the recombinant β helical protein is linked to a component selected from the group consisting of: one or more molecules of a genome-editing system and a plasmid that encodes one or more molecules of a genome-editing system, wherein the recombinant β helical protein length is in the range of from 5 to 25 nanometers, and width is in the range from 1 to 5 nanometers.