US 12,331,303 B2
Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair
Peter R. Beetham, Carlsbad, CA (US); Gregory F. W. Gocal, San Diego, CA (US); Christian Schopke, Carlsbad, CA (US); Noel Joy Sauer, Oceanside, CA (US); James Pearce, La Jolla, CA (US); Rosa E. Segami, Escondido, CA (US); and Jerry Mozoruk, Encinitas, CA (US)
Assigned to CIBUS US LLC, San Diego, CA (US); and CIBUS EUROPE B.V., Breda (NL)
Filed by CIBUS US LLC, San Diego, CA (US); and CIBUS EUROPE B.V., Breda (NL)
Filed on Aug. 22, 2022, as Appl. No. 17/892,967.
Application 17/892,967 is a continuation of application No. 16/410,226, filed on May 13, 2019, granted, now 11,421,240.
Application 16/410,226 is a continuation of application No. 14/777,357, granted, now 10,287,594, issued on May 14, 2019, previously published as PCT/US2014/029566, filed on Mar. 14, 2014.
Claims priority of provisional application 61/801,333, filed on Mar. 15, 2013.
Prior Publication US 2023/0313211 A1, Oct. 5, 2023
Int. Cl. C12N 15/82 (2006.01); C12N 9/22 (2006.01)
CPC C12N 15/8213 (2013.01) [C12N 9/22 (2013.01); C12N 15/8275 (2013.01); C12N 2800/80 (2013.01)] 3 Claims
 
1. A method for introducing a gene repair oligonucleobase (GRON)-mediated mutation into a target genomic deoxyribonucleic acid (DNA) sequence in a plant cell, comprising:
delivering into the plant cell one or more site-specific nucleases which induce single strand nicks or double DNA strand breaks into the plant cell genome and a GRON configured to mediate introduction of one or more targeted genetic changes within the plant cell genome, wherein
the GRON is greater than 55 bases in length, and
the GRON comprises one or more intrastrand crosslinks; and
selecting a plant cell comprising the one or more targeted genetic changes within the DNA sequence in the plant cell genome.