	US 12,331,301 B2
	Mutant strains of Trichoderma reesei
Frédérique Bidard-Michelot, L'Etang la Ville (FR); and Antoine Margeot, Paris (FR)
Assigned to IFP Energies Nouvelles, Rueil Malmaison (FR)
Appl. No. 16/063,954
Filed by IFP Energies Nouvelles, Rueil Malmaison (FR)
PCT Filed Dec. 21, 2016, PCT No. PCT/FR2016/053601 § 371(c)(1), (2) Date Jun. 19, 2018, PCT Pub. No. WO2017/115033, PCT Pub. Date Jul. 6, 2017.
Claims priority of application No. 1563382 (FR), filed on Dec. 28, 2015.
Prior Publication US 2020/0270619 A1, Aug. 27, 2020
Int. Cl. C12N 15/80 (2006.01); C07K 14/37 (2006.01); C12N 1/14 (2006.01); C12N 9/42 (2006.01); C12N 15/67 (2006.01); C12R 1/885 (2006.01)

CPC C12N 15/80 (2013.01) [C07K 14/37 (2013.01); C12N 15/67 (2013.01); C12N 1/145 (2021.05); C12N 9/2437 (2013.01); C12R 2001/885 (2021.05)]

5 Claims

1. A method of increasing the expression of a cellulase enzyme in a strain of a filamentous fungus, comprising

culturing a strain overexpressing a TrAZF1 gene, and collecting supernatant containing the cellulase enzyme from the strain culture;

wherein the strain genome contains a cassette comprising:

(a) at least one constitutive promoter which induces overexpression of SEQ ID NO:1 or SEQ ID NO:3 during strain culture;

(b) a nucleotide sequence of SED ID NO:1 or SEQ ID NO:3; and