	US 12,331,295 B2
	Modifying the specificity of plant non-coding RNA molecules for silencing gene expression
Eyal Maori, Cambridge (GB); Yaron Galanty, Cambridge (GB); Cristina Pignocchi, Norwich (GB); Angela Chaparro Garcia, Norwich (GB); and Ofir Meir, Norwich (GB)
Assigned to TROPIC BIOSCIENCES UK LIMITED, Norwich (GB)
Filed by Tropic Biosciences UK Limited, Norwich (GB)
Filed on Jan. 10, 2023, as Appl. No. 18/152,718.
Application 18/152,718 is a division of application No. 16/648,748, granted, now 11,555,199, previously published as PCT/IB2018/057160, filed on Sep. 18, 2018.
Claims priority of application No. 1715113 (GB), filed on Sep. 19, 2017; application No. 1715116 (GB), filed on Sep. 19, 2017; and application No. 1719516 (GB), filed on Nov. 23, 2017.
Prior Publication US 2023/0279414 A1, Sep. 7, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/11 (2006.01); C12N 9/22 (2006.01); C12N 15/113 (2010.01); C12N 15/82 (2006.01); C12N 15/85 (2006.01); C12N 15/90 (2006.01)

CPC C12N 15/113 (2013.01) [C12N 9/22 (2013.01); C12N 15/8213 (2013.01); C12N 15/8279 (2013.01); C12N 15/85 (2013.01); C12N 15/902 (2013.01); C12N 15/8218 (2013.01); C12N 2310/11 (2013.01); C12N 2310/141 (2013.01); C12N 2310/20 (2017.05)]

19 Claims

1. A method of modifying a gene encoding or processed into a non-coding RNA molecule having no RNA silencing activity in a plant cell, the method comprising introducing into the plant cell a DNA editing agent conferring a silencing specificity of said non-coding RNA molecule towards a target RNA of interest, thereby modifying the gene encoding or processed into the non-coding RNA molecule.