US 12,331,289 B2
Screening artificial nucleic acids by particle display
John Chaput, Irvine, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Filed by THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
Filed on Feb. 13, 2023, as Appl. No. 18/168,066.
Application 18/168,066 is a continuation of application No. 16/864,703, filed on May 1, 2020, granted, now 11,591,593.
Claims priority of provisional application 62/842,022, filed on May 2, 2019.
Prior Publication US 2023/0193253 A1, Jun. 22, 2023
Int. Cl. C12N 15/115 (2010.01); C12N 15/10 (2006.01); C12Q 1/68 (2018.01)
CPC C12N 15/1093 (2013.01) [C12N 15/115 (2013.01); C12N 2310/16 (2013.01)] 5 Claims
 
1. A method of screening for an XNA aptamer having a desired property, comprising the steps of:
(a) obtaining a monoclonal xeno-nucleic acid aptamer particle (mXNAP) display library, comprising at least 105 mXNAP display particles comprising a plurality of nucleic acid molecules comprising a dsDNA region and a single-stranded xeno-nucleic acid (XNA) aptamer region, wherein the mXNAP library is selected from the group consisting of a monoclonal threose nucleic acid aptamer particle (mTNAP), monoclonal hexitol nucleic acid aptamer particle (mHNAP), monoclonal cyclohexene nucleic acid aptamer particle (mCeNAP), monoclonal locked nucleic acid aptamer particle (mLNAP), monoclonal arabino nucleic acid aptamer particle (mANAP), monoclonal alkyl phosphonate nucleic acid aptamer particle (mphNAP), and monoclonal 2′-deoxy-2′-fluoroarabinonucleic acid aptamer particle (mFANAP) library,
(b) incubating a mXNAP display library with at least one candidate interaction partner for an amount of time sufficient for interaction of the XNA aptamer regions with the candidate interaction partner; and
(c) selecting mXNAP particles displaying XNA aptamers that have the desired property.