US 12,331,286 B2
Methods of adding polymers to ribonucleic acids
Manuel Ares, Jr., Santa Cruz, CA (US); Logan Mulroney, Santa Cruz, CA (US); Jenny Vo, Santa Cruz, CA (US); and Mark Akeson, Santa Cruz, CA (US)
Assigned to The Regents of the University of California
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Feb. 26, 2024, as Appl. No. 18/587,665.
Application 18/587,665 is a division of application No. 16/886,398, filed on May 28, 2020, granted, now 11,926,819.
Claims priority of provisional application 62/853,567, filed on May 28, 2019.
Prior Publication US 2024/0247251 A1, Jul. 25, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/12 (2006.01); C12N 15/10 (2006.01); C12Q 1/6809 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6876 (2018.01)
CPC C12N 15/102 (2013.01) [C12N 9/1282 (2013.01); C12Y 207/07052 (2013.01); C12Y 207/07072 (2013.01)] 8 Claims
 
1. A method of adding a polymer of non-canonical nucleotides to the 3′ end of a non-polyadenylated ribonucleic acid (RNA), comprising combining:
a non-polyadenylated RNA;
a Schizosaccharomyces pombe cid1 (S. pombe cid1) uridylyltransferase; and
non-canonical nucleotides,
in a reaction mixture under conditions in which the S. pombe cid1 uridylyltransferase adds the non-canonical nucleotides to the 3′ end of the non-polyadenylated RNA, thereby adding a polymer of non-canonical nucleotides to the 3′ end of the non-polyadenylated RNA.