US 12,331,284 B2
Coupled sorting and electric treatment of biological cells
Klemens Wassermann, Korneuburg (AT); Terje Wimberger, Vienna (AT); and Johannes Peham, Vienna (AT)
Assigned to AIT AUSTRIAN INSTITUTE OF TECHNOLOGY GMBH, Vienna (AT)
Appl. No. 17/299,476
Filed by AIT AUSTRIAN INSTITUTE OF TECHNOLOGY GMBH, Vienna (AT)
PCT Filed Dec. 12, 2019, PCT No. PCT/EP2019/084790
§ 371(c)(1), (2) Date Jun. 3, 2021,
PCT Pub. No. WO2020/120650, PCT Pub. Date Jun. 18, 2020.
Claims priority of application No. 18211954 (EP), filed on Dec. 12, 2018.
Prior Publication US 2022/0049208 A1, Feb. 17, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 13/00 (2006.01); C12M 1/00 (2006.01); C12M 1/42 (2006.01); C12Q 3/00 (2006.01)
CPC C12N 13/00 (2013.01) [C12M 35/02 (2013.01); C12M 47/04 (2013.01); C12M 47/06 (2013.01); C12Q 3/00 (2013.01)] 31 Claims
 
1. A method for lysis or electroporation of cells in a biological sample comprising the following steps:
passing cells of the sample, through a flow path with a flow speed, wherein the flow path runs through a detection apparatus for detecting individual cells and wherein the flow path comprises an array of two or more segments of the flow path, each said segment of the array comprising at least two electrodes for generating an electric field, and which electrodes are located downstream of the detection apparatus and which electrodes are coated with a dielectric material with a relative permittivity greater than 3.9, wherein the coating at least covers the surface of the electrodes that faces the flow path; and
when the presence of a specific cell is detected in the detection apparatus, then generating an electric field between the electrodes when the detected cell passes between the electrodes in dependence of the flow speed, wherein the array of segments generates an array of electric fields, wherein the electric fields cause electroporation or lysis of the cell.