US 12,331,281 B2
Method for directing cell adhesion and growth using ultrathin striped molecular films
Shelley A. Claridge, Lafayette, IN (US); Tyson C. Davis, Lafayette, IN (US); Sarah Calve, West Lafayette, IN (US); Alita Miller, West Lafayette, IN (US); and Anamika Singh, West Lafayette, IN (US)
Assigned to Purdue Research Foundation, West Lafayette, IN (US)
Filed by Purdue Research Foundation, West Lafayette, IN (US)
Filed on Sep. 3, 2021, as Appl. No. 17/466,048.
Claims priority of provisional application 63/073,961, filed on Sep. 3, 2020.
Prior Publication US 2022/0064586 A1, Mar. 3, 2022
Int. Cl. C12N 1/04 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12N 5/00 (2006.01); C08L 83/04 (2006.01)
CPC C12N 1/04 (2013.01) [C12M 23/04 (2013.01); C12M 23/20 (2013.01); C12M 25/06 (2013.01); C12N 5/0068 (2013.01); C08L 83/04 (2013.01); C12N 2513/00 (2013.01); C12N 2533/30 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A surface-functionalized cell culture support, wherein the surface of the support material is modified by a transferred monolayer of polymerized striped phases of molecules comprising one or more chemical functional groups, one or more long alkyl chains, and one or more polymerizable functional groups; wherein said transferred striped monolayer is useful for modulating directional adhesion, proliferation, differentiation, or reprogramming of a cell wherein the spatially controlled arrangement of chemical functional groups of the striped phase occurs at a sub-10-nm scale for the spatial placement of those functional elements, wherein the spatial arrangement of the chemical functional groups of the striped monolayer mimic properties of polysaccharide components of an extracellular matrix useful for modulating adhesion, proliferation, differentiation, or reprogramming of a cell and wherein said chemical functional groups comprise a carbohydrate, Arg-Gly-Asp peptide or a functional analog thereof, a matrisome component, or a combination thereof.