US 12,331,280 B2
Cell culture device, method for culturing cells, and method for producing product
Naoto Takahashi, Kanagawa (JP); Shinichi Nakai, Kanagawa (JP); and Hiroshi Sakuyama, Kanagawa (JP)
Assigned to FUJIFILM Corporation, Tokyo (JP)
Filed by FUJIFILM Corporation, Tokyo (JP)
Filed on Jul. 28, 2021, as Appl. No. 17/387,091.
Application 17/387,091 is a continuation of application No. PCT/JP2020/001094, filed on Jan. 15, 2020.
Claims priority of application No. 2019-018245 (JP), filed on Feb. 4, 2019.
Prior Publication US 2021/0355430 A1, Nov. 18, 2021
Int. Cl. C12N 1/02 (2006.01); C12M 1/00 (2006.01); C12N 5/071 (2010.01); F04B 43/00 (2006.01)
CPC C12N 1/02 (2013.01) [C12M 29/00 (2013.01); C12N 5/0682 (2013.01); F04B 43/0054 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A cell culture device comprising:
a culture vessel containing a cell suspension; and
a diaphragm pump for extracting the cell suspension from the culture vessel,
wherein a volume of a space defined by a diaphragm of the diaphragm pump and a plane F including a surface of an outer edge part of the diaphragm on a side far from an apex of the diaphragm is 1 cm3 or more and 20 cm3 or less,
in a case where an angle formed by a straight line connecting a foot of a perpendicular line which is drawn from the apex of the diaphragm to the plane F to each point in the diaphragm and by the perpendicular line is denoted by an angle A at each point, a thickness H of the diaphragm is within a range of 0.5 mm or more and 1.5 mm or less at any point in a region where the angle A is from 0° to 75°,
in a case where a thickness of the diaphragm at the apex of the diaphragm is denoted by HT, the thickness H of the diaphragm satisfies Expression 1 at any point in the region where the angle A is from 0° to 75°,
1≤HT/H≤1.75  (Expression 1), and
wherein in a case where a thickness of the diaphragm at a thickest point among points in a region where the angle A of the diaphragm is from 75° to 88° is HY and a thickness of the diaphragm at a point where the angle A is 90° is HZ, HZ and HY satisfy HZ<HY, and
the thickness H of the diaphragm satisfies H≤HZ at any point in the region where the angle A is from 0° to 75°.