US 12,331,278 B2
Continuous monitoring of algae crops using minimum optical information
Ilan Koren, Rehovot (IL); Assaf Vardi, Rehovot (IL); Yinon Rudich, Rehovot (IL); Adi Volpert, Rehovot (IL); and Joseph Bolless, Rehovot (IL)
Assigned to Yeda Research and Development Co. Ltd., Rehovot (IL)
Filed by Yeda Research and Development Co. Ltd., Rehovot (IL)
Filed on Sep. 14, 2021, as Appl. No. 17/474,208.
Application 17/474,208 is a continuation of application No. PCT/IL2020/050298, filed on Mar. 12, 2020.
Prior Publication US 2021/0403854 A1, Dec. 30, 2021
Int. Cl. C12M 1/34 (2006.01); C12Q 1/02 (2006.01); G01N 21/31 (2006.01); G01N 21/64 (2006.01)
CPC C12M 41/46 (2013.01) [C12Q 1/02 (2013.01); G01N 21/31 (2013.01); G01N 21/6486 (2013.01)] 24 Claims
OG exemplary drawing
 
1. A method for monitoring species of algae for stress comprising:
growing a test set of algae of a first species;
applying a stress of a predetermined kind to some of said algae, remaining algae being unstressed;
irradiating said algae at a predetermined first set of irradiation wavelengths; said first set comprising a plurality of wavelengths;
monitoring said algae at a predetermined second set of fluorescence/absorbance wavelengths to detect fluorescence and/or absorbance of said stressed algae and fluorescence and/or absorbance of said unstressed algae, said second set comprising a plurality of wavelengths;
comparing detected fluorescence and/or absorbance for each irradiation wavelength between said stressed algae and unstressed algae to find signs indicating said applied stress;
generating a plurality of combinations of respective irradiation wavelengths and fluorescence/absorbance wavelengths, wherein some of said combinations indicate said applied stress better than others of said combinations;
searching through said combinations of respective irradiation wavelengths and detected wavelengths to find a minimal set of irradiating and detected wavelengths that detects said applied stress to a required level of reliability; and
using said minimal set, irradiating further sets of algae of said first species to detect stress of said predetermined kind.