US 12,331,277 B2
Method and system for automated microbial colony counting from streaked sample on plated media
Timothy M. Wiles, Ocean Isle Beach, NC (US); and Raphael R. Marcelpoil, Corenc (FR)
Assigned to BD KIESTRA B.V., Drachten (NL)
Filed by BD KIESTRA B.V., Drachten (NL)
Filed on May 3, 2023, as Appl. No. 18/142,764.
Application 16/876,518 is a division of application No. 15/567,427, granted, now 10,696,938, issued on Jun. 30, 2020, previously published as PCT/US2016/028919, filed on Apr. 22, 2016.
Application 18/142,764 is a continuation of application No. 16/876,518, filed on May 18, 2020, granted, now 11,674,116.
Claims priority of provisional application 62/318,488, filed on Apr. 5, 2016.
Claims priority of provisional application 62/151,688, filed on Apr. 23, 2015.
Prior Publication US 2023/0383232 A1, Nov. 30, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 1/34 (2006.01); C12Q 1/02 (2006.01); G06T 7/00 (2017.01); G06V 20/69 (2022.01)
CPC C12M 41/36 (2013.01) [C12Q 1/02 (2013.01); G06T 7/0016 (2013.01); G06V 20/695 (2022.01); G06V 20/698 (2022.01); G06T 2207/10056 (2013.01); G06T 2207/30024 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A computer-readable memory storage medium having program instructions encoded thereon configured to cause a processor to perform a method of estimating a number of colony forming units on plated culture media, the method comprising:
at a predetermined incubation time of the plated culture media inoculated with a biological sample according to a predefined pattern, instructing an image acquisition device to obtain a digital image of the plated culture media;
from the digital image, identifying colony candidates in the image;
linearizing the digital image according to the predefined pattern;
plotting the colony candidates according to pixels of linearized coordinates of the digital image; and
estimating the number of colony forming units on the plated culture media based on pixels of the colony candidates in the linearized digital image;
estimating an initial bead load of a magnetically controlled bead from the plot of colony candidates, wherein estimating the initial bead load comprises:
selecting a distance from origin along the main axis of the linearized image;
determining a probability that a colony forming unit is released by the bead at the selected distance;
counting the number of colony forming units present in the digital image that are farther from origin along the main axis than the selected distance; and,
wherein the estimated initial bead load is equal to a ratio between the determined probability and the counted number of colony forming units.