US 12,331,108 B2
Antibodies with functional domains in the elbow region between variable and constant domain
Antonio Lanzavecchia, Porza (CH); and Luca Piccoli, Bellinzona (CH)
Assigned to Institute for Research in Biomedicine, Bellinzona (CH)
Appl. No. 16/635,506
Filed by INSTITUTE FOR RESEARCH IN BIOMEDICINE, Bellinzona (CH)
PCT Filed Jul. 30, 2018, PCT No. PCT/EP2018/070640
§ 371(c)(1), (2) Date Jan. 30, 2020,
PCT Pub. No. WO2019/025391, PCT Pub. Date Feb. 7, 2019.
Claims priority of application No. PCT/EP2017/069357 (WO), filed on Jul. 31, 2017.
Prior Publication US 2020/0325220 A1, Oct. 15, 2020
Int. Cl. C07K 16/24 (2006.01); C07K 16/10 (2006.01); C07K 16/12 (2006.01); C07K 16/16 (2006.01)
CPC C07K 16/243 (2013.01) [C07K 16/1018 (2013.01); C07K 16/1027 (2013.01); C07K 16/1282 (2013.01); C07K 16/16 (2013.01); C07K 2317/31 (2013.01); C07K 2317/569 (2013.01); C07K 2317/622 (2013.01); C07K 2318/10 (2013.01); C07K 2319/01 (2013.01); C07K 2319/30 (2013.01)] 50 Claims
 
1. An antibody, or antigen binding fragment thereof, comprising:
(a) a first polypeptide chain comprising, in N- to C-terminal direction:
one or more variable domains,
a first polypeptide chain functional domain, and
one or more heavy chain constant domains; and
(b) a second polypeptide chain comprising, in N- to C-terminal direction:
one or more variable domains forming antigen binding sites with the one or more variable domains of the first polypeptide chain, and
one or more light chain constant domains,
wherein all amino acids required for or involved in functionality of said first polypeptide chain functional domain are contained in the first polypeptide chain functional domain and no fragments or amino acids of the second polypeptide chain are required for or involved in the functionality of the first polypeptide chain functional domain,
wherein the first polypeptide chain functional domain comprises or consists of an Ig-like domain, an scFv, or a VHH, and
wherein the first polypeptide functional domain is N-terminal to the most N-terminal heavy chain constant domain of the first polypeptide chain.