US 12,331,095 B2
Inducible T cell receptors and uses thereof
Slavoljub Milosevic, Munich (DE); Adriana Turqueti Neves, Olching (DE); Kristina Schoedel, Munich (DE); and Alexander Schmidt, Zangberg (DE)
Assigned to MEDIGENE IMMUNOTHERAPIES GMBH, Planegg-Martinsried (DE)
Appl. No. 16/980,345
Filed by MEDIGENE IMMUNOTHERAPIES GMBH, Planegg-Martinsried (DE)
PCT Filed Mar. 13, 2019, PCT No. PCT/EP2019/056228
§ 371(c)(1), (2) Date Sep. 11, 2020,
PCT Pub. No. WO2019/175209, PCT Pub. Date Sep. 19, 2019.
Claims priority of application No. 18161766 (EP), filed on Mar. 14, 2018.
Prior Publication US 2021/0038647 A1, Feb. 11, 2021
Int. Cl. C07K 14/725 (2006.01); A61K 31/138 (2006.01); A61K 40/11 (2025.01); A61K 40/32 (2025.01); A61K 40/42 (2025.01); C12N 15/86 (2006.01)
CPC C07K 14/7051 (2013.01) [A61K 31/138 (2013.01); A61K 40/11 (2025.01); A61K 40/32 (2025.01); A61K 40/4269 (2025.01); A61K 40/427 (2025.01); C12N 15/86 (2013.01); C12N 2799/027 (2013.01)] 19 Claims
 
1. A combination comprising one or more nucleic acid molecules, said one or more nucleic acid molecules comprising:
(a) a nucleic acid sequence A, comprising
(i) a nucleic acid sequence encoding an amino acid sequence being at least 90% identical to SEQ ID NO: 2 and optionally comprising at least one amino acid substitution compared to the amino acid sequence of SEQ ID NO: 2 selected from the group consisting of T44A and T47A, and
(ii) a nucleic acid sequence encoding an inducible dimerization domain linked to, and downstream of, said nucleic acid sequence of (a)(i); and
(b) a nucleic acid sequence B, comprising
(i) a nucleic acid sequence encoding an amino acid sequence being at least 90% identical to SEQ ID NO: 4 and comprising at least one amino acid substitution compared to the amino acid sequence of SEQ ID NO: 4 selected from the group consisting of K4V, N5P, Y37K, L63A, S77A and R79A, and
(ii) a nucleic acid sequence encoding an inducible dimerization domain linked to, and downstream of, said nucleic acid of (b)(ii), wherein said dimerization domain corresponds to the dimerization domain of (a)(ii),
wherein said amino acid sequence of (a)(i) comprises at least one amino acid substitution compared to SEQ ID NO: 2.