US 12,331,073 B2
Preparation of biologically active complexes
Aftab Nadeem, Lund (SE); Catharina Svanborg, Malmo (SE); and Chin Shing Ho, Lund (SE)
Assigned to HAMLET PHARMA AB, Lund (SE)
Filed by HAMLET PHARMA AB, Lund (SE)
Filed on Jan. 12, 2021, as Appl. No. 17/146,588.
Application 17/146,588 is a division of application No. 16/612,272, abandoned, previously published as PCT/EP2018/062396, filed on May 14, 2018.
Claims priority of application No. 1707715 (GB), filed on May 14, 2017.
Prior Publication US 2021/0130394 A1, May 6, 2021
Int. Cl. C07K 1/107 (2006.01); A61K 38/00 (2006.01); A61P 35/00 (2006.01); C07K 1/34 (2006.01); C07K 14/76 (2006.01)
CPC C07K 1/107 (2013.01) [A61P 35/00 (2018.01); C07K 1/34 (2013.01); C07K 14/76 (2013.01); A61K 38/00 (2013.01)] 11 Claims
 
1. A method for preparing a biologically active complex, said method comprising dissolving a mixture of a polypeptide element in powder form and oleic acid or a pharmaceutically acceptable salt thereof also in solid form, in an aqueous solvent comprising at least two salts to form an aqueous solution comprising a biologically active complex of the polypeptide element and oleic acid, wherein the first salt of the at least two salts is sodium chloride or potassium chloride and the second salt of the at least two salts is disodium phosphate or mono-potassium phosphate, wherein the method is performed at a moderate temperature of up to 50° C., and wherein the polypeptide element is:
(a) an alpha-lactalbumin or an alpha-lactalbumin having each of the cysteine residues replaced with another amino acid;
(b) a peptide fragment of an alpha-lactalbumin having up to 50 amino acids and comprising SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:6 or SEQ ID NO:7;
(c) a peptide fragment of a Coat Protein Complex II (COPII) family protein, wherein the peptide fragment has up to 50 amino acids and comprises SEQ ID NO:5; or
(d) a lysozyme.