US 12,330,139 B2
Method for extracting low-molecular-weight substance existing in biological sample
Yoshiyuki Koga, Fukuoka (JP)
Assigned to TAS PROJECT CO. LTD, Fukuoka (JP)
Appl. No. 17/789,445
Filed by TAS PROJECT CO. LTD., Fukuoka (JP)
PCT Filed Dec. 21, 2020, PCT No. PCT/JP2020/047735
§ 371(c)(1), (2) Date Jun. 27, 2022,
PCT Pub. No. WO2021/132178, PCT Pub. Date Jul. 1, 2021.
Claims priority of application No. 2019-236585 (JP), filed on Dec. 26, 2019.
Prior Publication US 2023/0029620 A1, Feb. 2, 2023
Int. Cl. B01J 20/283 (2006.01); B01D 15/08 (2006.01); B01J 20/20 (2006.01); B01J 20/34 (2006.01); G01N 30/06 (2006.01); G01N 33/48 (2006.01)
CPC B01J 20/283 (2013.01) [B01D 15/08 (2013.01); B01J 20/20 (2013.01); B01J 20/34 (2013.01); G01N 30/06 (2013.01); G01N 33/48 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method for extracting a low-molecular-weight substance existing in a biological sample from the sample, the method comprising:
1) an adsorption step of adsorbing the low-molecular-weight substance on porous carbon by mixing the biological sample with the porous carbon having mesopores of 3.5 nm to 150 nm and micropores of a larger size as a hierarchical structure, and then recovering the porous carbon from the obtained mixture, or by bringing the biological sample into contact with a filtration filter on which the porous carbon having mesopores of 3.5 nm to 150 nm and micropores of a larger size as a hierarchical structure is disposed or supported; and
2) a releasing step of releasing the low-molecular-weight substance from the porous carbon by mixing the porous carbon having the low-molecular-weight substance adsorbed thereon, obtained after the adsorption step, with an aqueous solution containing 0.1 mass % to 1 mass % of spherical silica having an average particle diameter of 10 nm to 100 nm and containing 10% to 12% of acetonitrile, or by causing the filtration filter obtained after the adsorption step to contact and pass through the aqueous solution.