US 12,329,857 B2
Systems and methods for manufacturing lipid nanoparticles and liposomes
Christopher J. Barbosa, Coquitlam (CA); Kody Moleschi, Delta (CA); and David Cretney, Vancouver (CA)
Assigned to Acuitas Therapeutics, Inc., Vancouver (CA)
Appl. No. 17/277,691
Filed by ACUITAS THERAPEUTICS, INC., Vancouver (CA)
PCT Filed Sep. 20, 2019, PCT No. PCT/US2019/052105
§ 371(c)(1), (2) Date Mar. 18, 2021,
PCT Pub. No. WO2020/061426, PCT Pub. Date Mar. 26, 2020.
Claims priority of provisional application 62/734,837, filed on Sep. 21, 2018.
Prior Publication US 2022/0273567 A1, Sep. 1, 2022
Int. Cl. A61K 9/1272 (2025.01); A61K 9/51 (2006.01); A61K 31/7105 (2006.01); B01F 23/45 (2022.01); B01F 25/23 (2022.01); B01F 35/71 (2022.01); B01F 35/83 (2022.01)
CPC A61K 9/1272 (2013.01) [A61K 9/5146 (2013.01); A61K 31/7105 (2013.01); B01F 23/45 (2022.01); B01F 25/23 (2022.01); B01F 35/71805 (2022.01); B01F 35/834 (2022.01)] 24 Claims
OG exemplary drawing
 
1. A system, comprising:
a first reservoir, the first reservoir containing an organic lipid stock solution;
a first pump having a first pump inlet and a first pump outlet, the first pump inlet fluidically coupled to the first reservoir;
a second reservoir, the second reservoir containing an aqueous nucleic acid stock solution;
a second pump having a second pump inlet and a second pump outlet, the second pump inlet fluidically coupled to the second reservoir;
a valve having a first valve inlet port fluidically coupled to the first pump outlet, a second valve inlet port fluidically coupled to the second pump outlet, a first valve outlet port, a second valve outlet port, a third valve outlet port, and a fourth valve outlet port; and
a mixing assembly having a first mixing assembly inlet fluidically coupled to the first valve outlet port, a second mixing assembly inlet fluidically coupled to the second valve outlet port, and a mixing assembly outlet, wherein the first mixing assembly inlet is fluidically coupled to the mixing assembly outlet and the second mixing assembly inlet is fluidically coupled to the mixing assembly outlet;
wherein the valve has a first operating position in which the first valve inlet port is fluidically coupled to the first valve outlet port and the second valve inlet port is fluidically coupled to the second valve outlet port and a second operating position in which the first valve inlet port is not fluidically coupled to the first valve outlet port and the second valve inlet port is not fluidically coupled to the second valve outlet port,
wherein the third valve outlet port is fluidically coupled to the first reservoir in both of the first and second operating positions, and
wherein the fourth valve outlet port is fluidically coupled to the second fluid reservoir in both of the first and second operating positions.