US 12,329,813 B2
Porcine epidemic diarrhea virus strains and immunogenic compositions therefrom
Jianqiang Zhang, Ames, IA (US); Qi Chen, Ames, IA (US); Phillip Gauger, Ames, IA (US); Darin Madson, Ames, IA (US); John Morgan Hardham, Parsippany, NJ (US); Meggan Bandrick, Parsippany, NJ (US); Jay Gregory Calvert, Parsippany, NJ (US); James Richard Thompson, Parsippany, NJ (US); Mira Ivanova Stoeva, Parsippany, NJ (US); and Walter Valdez, Parsippany, NJ (US)
Assigned to Zoetis Services LLC, Parsippany, NJ (US); and Iowa State University Research Foundation, Inc., Ames, IA (US)
Filed by IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC., Ames, IA (US); and ZOETIS SERVICES LLC, Parsippany, NJ (US)
Filed on May 10, 2024, as Appl. No. 18/660,609.
Application 18/660,609 is a continuation of application No. 17/021,261, filed on Sep. 15, 2020, granted, now 12,005,113.
Application 17/021,261 is a continuation of application No. 15/556,446, granted, now 11,058,763, issued on Jul. 13, 2021, previously published as PCT/US2016/019846, filed on Feb. 26, 2016.
Claims priority of provisional application 62/276,022, filed on Jan. 7, 2016.
Claims priority of provisional application 62/250,961, filed on Nov. 4, 2015.
Claims priority of provisional application 62/209,119, filed on Aug. 24, 2015.
Claims priority of provisional application 62/121,955, filed on Feb. 27, 2015.
Prior Publication US 2025/0073330 A1, Mar. 6, 2025
Int. Cl. A61K 39/215 (2006.01); A61K 39/12 (2006.01); C07K 14/165 (2006.01); A61K 39/00 (2006.01)
CPC A61K 39/215 (2013.01) [A61K 39/12 (2013.01); C07K 14/165 (2013.01); A61K 2039/51 (2013.01); A61K 2039/552 (2013.01); C12N 2770/20034 (2013.01)] 9 Claims
 
1. A vaccine composition comprising an attenuated prototype passaged strain of PEDV, and a carrier, wherein said strain of PEDV is at least 95% identical, at a full length nucleotide level, to SEQ ID NO:67 and comprises a nucleic acid sequence which encodes:
a polyprotein 1a/1b with one or more substitutions at position 814, 1076, 1564, 1896, 2310, 2600, 3247, 3473, or 3522 as determined by reference to SEQ ID NO:52; and
a spike protein with one or more substitutions at position 257, 326, 375, 491, 881, 888, 1277, 1339, or 1358 as determined by reference to SEQ ID NO:54; and
an ORF3 protein truncated at position 39 or later as determined by reference to SEQ ID NO:55; and
an envelope protein with one or more substitutions at position 69 or 62 as determined by reference to SEQ ID NO:56; and
a membrane protein with a substitution at position 208 as determined by reference to SEQ ID NO: 57; and
a nucleocapsid protein with one or more substitutions at position 141, 418, 424, or 439 as determined by reference to SEQ ID NO: 58.