	US 12,329,810 B2
	Production of viruses in continuously growing epithelial cell lines derived from chicken gut
Michael Veit, Berlin (DE); Chris Tina Höfer, Berlin (DE); and Wolfgang Rudy, Karlsruhe (DE)
Assigned to FREIE UNIVERSITÄT BERLIN, Berlin (DE); and TENTAMUS PHARMA & MED DEUTSCHLAND GMBH, Karlsruhe (DE)
Appl. No. 17/426,043
Filed by FREIE UNIVERSITÄT BERLIN, Berlin (DE); and TENTAMUS PHARMA & MED DEUTSCHLAND GMBH, Karlsruhe (DE)
PCT Filed Jan. 28, 2020, PCT No. PCT/EP2020/052061 § 371(c)(1), (2) Date Jul. 27, 2021, PCT Pub. No. WO2020/157076, PCT Pub. Date Aug. 6, 2020.
Claims priority of application No. 19154039 (EP), filed on Jan. 28, 2019.
Prior Publication US 2022/0118078 A1, Apr. 21, 2022
Int. Cl. A61K 39/145 (2006.01); A61K 39/00 (2006.01); C12N 5/071 (2010.01); C12N 7/00 (2006.01)

CPC A61K 39/145 (2013.01) [C12N 5/0679 (2013.01); C12N 7/00 (2013.01); A61K 39/00 (2013.01); A61K 2039/552 (2013.01); C12N 2760/16151 (2013.01)]

13 Claims

1. A method for manufacturing viruses in an in vitro cell culture, comprising the following steps: a) providing a cell culture of avian epithelial cells chosen from at least one of the cell cultures deposited under deposition numbers DSM ACC3345, DSM ACC3346, DSM ACC3347, DSM ACC3348, DSM ACC3349; b) infecting at least some of the cells in the cell culture with virus particles; c) incubating the cells for a first period of time; and d) recovering viruses produced by the cell culture from a supernatant of the cell culture.