US 12,329,769 B2
Use of Abelmoschi corolla extract in preparation of drug for treating fibrosis
Haitao Tang, Taizhou (CN); Zhengyu Cao, Nanjing (CN); Boyang Yu, Nanjing (CN); Chengzhi Chai, Nanjing (CN); Haitao Ge, Taizhou (CN); and Zhengjun Wang, Taizhou (CN)
Assigned to SUZHONG PHARMACEUTICAL GROUP CO., LTD., Taizhou (CN); and JIANGSU SUZHONG PHARMACEUTICAL RESEARCH INSTITUTE CO., LTD., Nanjing (CN)
Appl. No. 17/596,413
Filed by SUZHONG PHARMACEUTICAL GROUP CO., LTD., Taizhou (CN); and JIANGSU SUZHONG PHARMACEUTICAL RESEARCH INSTITUTE CO., LTD., Nanjing (CN)
PCT Filed May 18, 2020, PCT No. PCT/CN2020/090897
§ 371(c)(1), (2) Date Dec. 9, 2021,
PCT Pub. No. WO2020/248776, PCT Pub. Date Dec. 17, 2020.
Claims priority of application No. 201910509730.7 (CN), filed on Jun. 13, 2019; and application No. 201910746618.5 (CN), filed on Aug. 14, 2019.
Prior Publication US 2022/0339177 A1, Oct. 27, 2022
Int. Cl. A61K 31/7048 (2006.01); A61K 31/407 (2006.01); A61K 36/185 (2006.01)
CPC A61K 31/7048 (2013.01) [A61K 31/407 (2013.01); A61K 36/185 (2013.01)] 17 Claims
 
1. An herbal extract for treating fibrosis comprising at least 15% of gossypetin-3-O-β-D-glucose-8-O-β-D-glucuronide and acortatarin A by weight, wherein the herbal extract is prepared by the following steps: taking an herbal, carrying out ethanol heating reflux extraction of the herbal, filtering, concentrating a filtrate, carrying out macroporous resin column chromatography, washing the column with water first, then eluting the column with 3% to 7% ethanol, then eluting the column with 9% to 15% ethanol, and collecting a 9% to 15% ethanol eluate in order to obtain the herbal extract; wherein the ethanol is 50% to 95% in the step of ethanol heating reflux extraction.