US 12,007,545 B2
Light sheet microscope and method for light sheet microscopy
Ralf Netz, Jena (DE); and Joerg Siebenmorgen, Jena (DE)
Assigned to Carl Zeiss Microscopy GmbH, Jena (DE)
Filed by Carl Zeiss Microscopy GmbH, Jena (DE)
Filed on Oct. 28, 2021, as Appl. No. 17/513,833.
Claims priority of application No. 102020128524.8 (DE), filed on Oct. 29, 2020.
Prior Publication US 2022/0137382 A1, May 5, 2022
Int. Cl. G02B 21/00 (2006.01); G02B 21/26 (2006.01); G02B 21/36 (2006.01); H04N 23/74 (2023.01)
CPC G02B 21/008 (2013.01) [G02B 21/0032 (2013.01); G02B 21/26 (2013.01); G02B 21/365 (2013.01); H04N 23/74 (2023.01)] 9 Claims
OG exemplary drawing
 
1. A light sheet microscope, comprising:
a sample volume, including a sample stage for a sample carrier with a sample;
a detection device comprising a camera that images the sample volume along an optical imaging axis;
an illumination device comprising cylindrical optics or a scanned laser beam which illumination device illuminates the sample with a light sheet, comprising a light sheet plane that is adjustable in the sample volume, wherein the light sheet plane is arranged substantially perpendicularly to the optical imaging axis and the light sheet has an illumination width extending perpendicular to the light sheet plane; and
a processing device comprising a computing unit and a wired or wireless, connection to the detection device and further to the illumination device or to the sample stage or to both, wherein the processing device is configured to:
control the illumination device or the sample stage or both to set a location of the light sheet plane sequentially to different locations of the light sheet plane of the light sheet in the sample volume,
control the detection device to record an image of the sample volume for each of the different locations of the light sheet plane, whereby a stack of a plurality of images of the sample volume is produced, with each image in the stack corresponding to a single one of the different locations of the light sheet plane,
control the illumination device or the sample stage or both such that for the plurality of images recorded a spacing, which is measured along the optical imaging axis, between adjacent locations of the different locations of the light sheet plane is greater than or equal to half the illumination width, but not greater than the illumination width, and
compute intermediate images for the stack, each of the intermediate images corresponding to a virtual additional location of the light sheet plane in the sample volume.