CPC G01N 33/6848 (2013.01) [G01N 33/6896 (2013.01); G01N 2458/15 (2013.01); G01N 2570/00 (2013.01)] | 24 Claims |
1. A method for measuring endogenous biomolecules in a biological sample, comprising:
(a) contacting a first aliquot of the biological sample with nanoparticles, thereby forming biomolecule coronas comprising the endogenous biomolecules of the biological sample adsorbed directly to the nanoparticles;
(b) measuring the contacted sample of (a) to obtain a first set of measurements;
(c) contacting a second aliquot of the biological sample with a plurality of reference biomolecules;
(d) measuring the contacted sample of (c) to obtain a second set of measurements; and
(e) combining the first and second sets of measurements, wherein the plurality of reference biomolecules comprises an isotopic label, a mass tag, a barcode, a post-translation modification, or a biomolecule from a species different than a species of a subject from whom the biological sample was taken.
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