US 12,007,397 B2
Enhanced detection and quantitation of biomolecules
Bruce Wilcox, Keezletown, VA (US); Kavya Swaminathan, Mountain View, CA (US); Preston B. Williams, San Mateo, WA (US); Jared Deyarmin, San Bruno, MA (US); and Mi Yang, Belmont, CA (US)
Assigned to PrognomiQ, Inc., San Mateo, CA (US)
Filed by PrognomiQ, Inc., San Mateo, CA (US)
Filed on Sep. 12, 2022, as Appl. No. 17/931,469.
Claims priority of provisional application 63/243,645, filed on Sep. 13, 2021.
Prior Publication US 2023/0089033 A1, Mar. 23, 2023
Int. Cl. G01N 33/68 (2006.01)
CPC G01N 33/6848 (2013.01) [G01N 33/6896 (2013.01); G01N 2458/15 (2013.01); G01N 2570/00 (2013.01)] 24 Claims
OG exemplary drawing
 
1. A method for measuring endogenous biomolecules in a biological sample, comprising:
(a) contacting a first aliquot of the biological sample with nanoparticles, thereby forming biomolecule coronas comprising the endogenous biomolecules of the biological sample adsorbed directly to the nanoparticles;
(b) measuring the contacted sample of (a) to obtain a first set of measurements;
(c) contacting a second aliquot of the biological sample with a plurality of reference biomolecules;
(d) measuring the contacted sample of (c) to obtain a second set of measurements; and
(e) combining the first and second sets of measurements, wherein the plurality of reference biomolecules comprises an isotopic label, a mass tag, a barcode, a post-translation modification, or a biomolecule from a species different than a species of a subject from whom the biological sample was taken.