CPC G01N 33/5695 (2013.01) [G01N 2800/065 (2013.01); G01N 2800/56 (2013.01)] | 10 Claims |
1. A method of treating Crohn's Disease in a subject in need thereof comprising the steps of:
a) obtaining a human blood or peripheral blood mononuclear cell (PBMC) sample from a subject diagnosed with Crohn's Disease;
b) lysing cells of the sample to release any viable target mycobacterial cell;
c) admixing a broad host range mycobacteriophage with the sample under conditions suitable to allow the bacteriophage to infect any viable target mycobacterial cell present in the sample;
d) removing any bacteriophage not infecting a viable target mycobacterial cell;
e) admixing at least a portion of the admixture of step c) following the removal of step d) with bacteria capable of supporting replication of the bacteriophage;
f) plating at least a portion of the admixture of step e) on a substrate to support growth of the bacteria;
g) quantifying the number of bacteriophage plaques that form;
h) discriminating between infected and asymptomatic subjects, comprising identifying the subject as infected when the number of bacteriophage plaques is statistically significantly greater than a reference number of bacteriophage plaques, or as asymptomatic when the number of bacteriophage plaques is not statistically significantly greater than a reference number of bacteriophage plaques, wherein the reference number of bacteriophage plaques is a reference number of plaques in asymptomatic human hosts; and
i) administering a pharmaceutical composition for inhibiting or disrupting microorganism growth or treating a microbial infection to a subject identified as infected.
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