US 12,007,391 B2
Means and methods for determining risk of type-1 diabetes by serum protein biomarkers
Robert Moulder, Turku (FI); Santosh Bhosale, Turku (FI); David Goodlett, Baltimore, MD (US); Harri Lähdesmäki, Espoo (FI); Olli Simell, Raisio (FI); and Riitta Lahesmaa, Turku (FI)
Assigned to TURUN YLIOPISTO, Turun Yliopisto (FI)
Filed by TURUN YLIOPISTO, Turun Yliopisto (FI)
Filed on Nov. 23, 2020, as Appl. No. 17/101,693.
Application 17/101,693 is a division of application No. 15/319,515, granted, now 10,877,034, previously published as PCT/FI2015/050448, filed on Jun. 18, 2015.
Claims priority of application No. 20140186 (FI), filed on Jun. 18, 2014.
Prior Publication US 2021/0080458 A1, Mar. 18, 2021
Int. Cl. G01N 31/00 (2006.01); G01N 33/53 (2006.01); G01N 33/564 (2006.01); G01N 33/92 (2006.01)
CPC G01N 33/564 (2013.01) [G01N 33/92 (2013.01); G01N 2333/47 (2013.01); G01N 2333/4713 (2013.01); G01N 2333/4716 (2013.01); G01N 2333/76 (2013.01); G01N 2333/775 (2013.01); G01N 2560/00 (2013.01); G01N 2570/00 (2013.01); G01N 2800/042 (2013.01); G01N 2800/50 (2013.01); G01N 2800/60 (2013.01)] 12 Claims
 
1. A method of determining an individual at risk of and/or progression towards Type 1 Diabetes (T1D), the method comprising:
a) determining a protein marker profile in a sample obtained from the individual, said profile comprising profilin-1 (PROF1) and filamin-A (FLNA),
b) comparing the determined protein marker profile of PROF1 and FLNA of the individual with that of a corresponding control profile of PROF1 and FLNA in a control sample from a control individual or a pool of control individuals,
c) responsive to the comparison, determining the risk of and/or progression towards T1D in the individual, wherein higher levels of PROF1 and FLNA in the sample obtained from the individual as compared to the levels of PROF1 and FLNA in the control sample indicates that the individual is at risk of and/or progression towards T1D, and
d) providing a treatment or dietary change to the individual to correct the protein marker profile of the individual towards that of an unaffected individual.