US 12,007,351 B2
Electrode-modified heavy metal ion microfluidic detection chip and preparation method
Ying Hong, Jiangsu (CN); Jiansong Chen, Jiangsu (CN); Juan Huang, Jiangsu (CN); Yangyun Wu, Jiangsu (CN); Lingling Tian, Jiangsu (CN); Wei Wang, Jiangsu (CN); Wei An, Jiangsu (CN); Jingling Wang, Jiangsu (CN); Yuanyuan Zhu, Jiangsu (CN); and Chen Tang, Jiangsu (CN)
Assigned to JIANGSU YANGTZE TESTING AND CERTIFICATION CO., LTD., Jiangsu (CN); and JINLING CUSTOMS TECHNNOLOGY CENTER, Jiangsu (CN)
Appl. No. 17/774,174
Filed by Jiangsu Yangtze Testing and Certification Co., Ltd., Jiangsu (CN); and Jinling Customs Technnology Center, Jiangsu (CN)
PCT Filed Mar. 27, 2020, PCT No. PCT/CN2020/081674
§ 371(c)(1), (2) Date May 4, 2022,
PCT Pub. No. WO2021/088304, PCT Pub. Date May 14, 2021.
Claims priority of application No. 201911064127.9 (CN), filed on Nov. 4, 2019.
Prior Publication US 2022/0349852 A1, Nov. 3, 2022
Int. Cl. G01N 27/30 (2006.01); B01L 3/00 (2006.01); B29C 64/112 (2017.01); B29C 64/30 (2017.01); B33Y 10/00 (2015.01); B33Y 40/20 (2020.01); B33Y 80/00 (2015.01); B41M 1/12 (2006.01); B41M 1/30 (2006.01); B41M 3/00 (2006.01); G01N 27/333 (2006.01); G01N 27/48 (2006.01); B29L 31/00 (2006.01)
CPC G01N 27/308 (2013.01) [B01L 3/502715 (2013.01); B29C 64/112 (2017.08); B29C 64/30 (2017.08); B33Y 10/00 (2014.12); B33Y 40/20 (2020.01); B33Y 80/00 (2014.12); B41M 1/12 (2013.01); B41M 1/30 (2013.01); B41M 3/006 (2013.01); G01N 27/333 (2013.01); G01N 27/48 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/16 (2013.01); B29L 2031/752 (2013.01)] 9 Claims
OG exemplary drawing
 
1. An electrode-modified heavy metal ion microfluidic detection chip, including a microfluidic module (1) and a three-electrode sensor (2), wherein:
the microfluidic module (1) comprises a micro-channel (10), an inlet duct (12) and an outlet duct (13) are provided at opposite ends of the at least one micro-channel (10); the three-electrode sensor (2) comprises three all-solid-state planar electrodes provided on a card-shaped base plate (20), a working electrode (21), an auxiliary electrode (22) and a reference electrode (23); one end of the three-electrode sensor (2) is an interface area (24), contacts (240) at terminals of leads of the three all-solid-state planar electrodes are arranged in the interface area; a sensor slot (11) matching the three-electrode sensor (2) is provided at a bottom portion of the micro-channel (10) in the microfluidic module (1); when the three-electrode sensor (2) is inserted in the sensor slot (11), the three all-solid-state planar electrodes are communicated with the micro-channel (10), the three-electrode sensor (2) is assembled with the microfluidic module (1) by being inserted into the sensor slot (11), and is detachable; the interface area (24) is outside the sensor slot (11);
the microfluidic module (1) is a 3D printed transparent flexible piece, the micro-channel (10), the inlet duct (12), the outlet duct (13), and the sensor slot (11) are integrally printed and formed on with the microfluidic module (1); the working electrode (21) is a bare carbon electrode, and a surface thereof is modified by porous nano-NiMn2O4; the auxiliary electrode (22) is an Ag electrode; and the reference electrode (23) is an Ag/AgCl electrode;
wherein preparing the porous nano-NiMn2O4, specific steps of preparing the porous nano-NiMn2O4 include:
dissolving MnCl2·6H2O 20 mmol/L, NiCl2·6H2O 40 mmol/L, Mn(NH2)2 120 mmol/L, and NH4F 0.1 g in ethanol 5 mL and deionized water 30 mL, stirring violently for 30 min;
cooling the solution to room temperature, cleaning reaction products with distilled water for at least 5 times, and drying the same; and
giving air annealing treatment to the reaction products at a tubular furnace at 2° C./min, preserving for 3 h at 350° C., so as to obtain porous nano-NiMn2O4 powder.