US 12,006,543 B2
Device preparation using condensed nucleic acid particles
Guobin Luo, Oceanside, CA (US); Marina Sedova, Foster City, CA (US); David Light, Branford, CT (US); Ryan Jones, Potomac, MD (US); and Mohammad Alanjary, Escondido, CA (US)
Assigned to LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed by LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed on Mar. 30, 2021, as Appl. No. 17/217,492.
Application 15/785,579 is a division of application No. 14/485,282, filed on Sep. 12, 2014, granted, now 9,797,011, issued on Oct. 24, 2017.
Application 17/217,492 is a continuation of application No. 16/741,704, filed on Jan. 13, 2020, abandoned.
Application 16/741,704 is a continuation of application No. 15/785,579, filed on Oct. 17, 2017, granted, now 10,533,220, issued on Jan. 14, 2020.
Claims priority of provisional application 62/020,292, filed on Jul. 2, 2014.
Claims priority of provisional application 61/877,745, filed on Sep. 13, 2013.
Prior Publication US 2021/0214787 A1, Jul. 15, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6874 (2018.01); C12Q 1/6806 (2018.01)
CPC C12Q 1/6874 (2013.01) [C12Q 1/6806 (2013.01)] 20 Claims
 
1. A method for depositing particles on a surface, the method comprising:
receiving a plurality of particles, a particle of the plurality of particles having a hydrogel polymer matrix conjugated to nucleic acid strands;
exposing the plurality of particles to a solution including:
a magnesium salt in a range of 30 mM to 500 mM;
a potassium salt in a range of 0.8 M to 1.0 M;
a buffering agent in a range of 150 mM to 500 mM; and
a surfactant in a range of 0.05% to 0.5%; and
applying the plurality of particles to a surface following exposing the plurality of particles to the solution, particles of the plurality of particles depositing on the surface.