US 12,006,542 B2
Force-modulated hybridization for visualizing nucleic acid length and function
Shoujun Xu, Houston, TX (US); Qiongzheng Hu, Houston, TX (US); and Yuhong Wang, Houston, TX (US)
Assigned to University of Houston System, Houston, TX (US)
Appl. No. 16/620,571
Filed by University of Houston System, Houston, TX (US)
PCT Filed Jun. 11, 2018, PCT No. PCT/US2018/036885
§ 371(c)(1), (2) Date Dec. 9, 2019,
PCT Pub. No. WO2018/227182, PCT Pub. Date Dec. 13, 2018.
Claims priority of provisional application 62/517,694, filed on Jun. 9, 2017.
Prior Publication US 2023/0160003 A1, May 25, 2023
Int. Cl. C12Q 1/6811 (2018.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); C12Q 1/6816 (2018.01); C12Q 1/6874 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6818 (2018.01)
CPC C12Q 1/6874 (2013.01) [B01L 3/502761 (2013.01); B01L 7/52 (2013.01); B01L 2200/0663 (2013.01); B01L 2300/044 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/0672 (2013.01); B01L 2300/18 (2013.01); B01L 2400/0439 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6818 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A method of determining the length of an analyte strand, comprising:
(a) incubating a magnetically labeled oligonucleotide strand, the analyte strand, and one of a series of oligonucleotide ruler strands to form a mixture,
wherein the magnetically labeled oligonucleotide strand is complementary in sequence to the analyte strand,
wherein the series of oligonucleotide ruler strands are complementary in sequence to the magnetically labeled oligonucleotide strand and comprise different lengths, and
wherein either the analyte strand or the oligonucleotide ruler strands are labeled with at least one label;
(b) transferring the mixture to a surface functionalized to couple with the at least one label;
(c) applying a mechanical force to the mixture; and
(d) inspecting the surface for immobilized particles,
wherein if the analyte strand is labeled, then the longest oligonucleotide ruler strand producing immobilized particles on the surface represents the length of the analyte strand, and
wherein if the oligonucleotide ruler strands are labeled, then the longest oligonucleotide ruler strand not producing immobilized particles represents the length of the analyte strand; and
(e) wherein a different oligonucleotide ruler strand is used to repeat steps (a)-(d) until the length of the analyte strand is determined.