US 12,006,536 B2
Chemically-enhanced primer compositions, methods and kits
Linda Lee, Palo Alto, CA (US); Sam Woo, Redwood City, CA (US); and Peter Ma, Cupertino, CA (US)
Assigned to LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed by LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed on Aug. 11, 2021, as Appl. No. 17/399,449.
Application 17/399,449 is a division of application No. 16/247,267, filed on Jan. 14, 2019, granted, now 11,104,944.
Application 16/247,267 is a division of application No. 15/652,224, filed on Jul. 17, 2017, granted, now 10,221,449, issued on Mar. 5, 2019.
Application 15/652,224 is a division of application No. 14/198,308, filed on Mar. 5, 2014, granted, now 9,708,650, issued on Jul. 18, 2017.
Application 14/198,308 is a continuation of application No. 13/397,626, filed on Feb. 15, 2012, granted, now 8,703,925, issued on Apr. 22, 2014.
Application 13/397,626 is a continuation in part of application No. 13/284,839, filed on Oct. 28, 2011, abandoned.
Claims priority of provisional application 61/408,553, filed on Oct. 29, 2010.
Claims priority of provisional application 61/407,899, filed on Oct. 28, 2010.
Prior Publication US 2022/0049289 A1, Feb. 17, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6853 (2018.01); C12Q 1/686 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6874 (2018.01)
CPC C12Q 1/686 (2013.01) [C12Q 1/6853 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6874 (2013.01); C12Q 2525/113 (2013.01); C12Q 2525/125 (2013.01)] 21 Claims
 
1. A chemically-enhanced primer, comprising:
a negatively charged moiety (NCM) disposed at a 5′ end of the chemically-enhanced primer, the NCM having a branched arrangement.