US 12,006,535 B2
Methods and devices for detecting SARS-COV-2
Inanc Ortac, San Diego, CA (US)
Assigned to SARMAL, INC., San Diego, CA (US)
Filed by Sarmal, Inc., San Diego, CA (US)
Filed on May 29, 2020, as Appl. No. 16/888,368.
Claims priority of provisional application 63/022,262, filed on May 8, 2020.
Claims priority of provisional application 62/937,634, filed on Nov. 19, 2019.
Prior Publication US 2021/0147907 A1, May 20, 2021
Int. Cl. C12Q 1/686 (2018.01); C12Q 1/6818 (2018.01); G01N 21/66 (2006.01)
CPC C12Q 1/686 (2013.01) [C12Q 1/6818 (2013.01); G01N 21/66 (2013.01); C12Q 2565/301 (2013.01)] 19 Claims
 
1. A method for enriching or isolating a target-nucleic acid from a nucleic acid-containing sample, said method comprising:
a) receiving an electrolytic fluid solution including ions and a nucleic acid-containing sample in a fluid chamber having a first and second electrode, wherein at least one electrode has attached thereto a plurality of capture-probes or capture/primer probes complementary to the target-nucleic acid, wherein the capture probes are at the electrode-solution interface;
b) applying an annealing-voltage to the electrolytic fluid in proximity to the electrode-solution interface to generate an annealing-pH level by the interface, wherein the annealing-pH level causes annealing of any number of complementary base pairs between nucleic acid within the nucleic acid-containing sample and the capture-probes or capture/primer probes;
c) applying a denaturing-voltage to the electrolytic fluid in proximity to the electrode-solution interface to generate a denaturing-pH level by the interface, wherein the denaturing-pH level causes the denaturation of double-stranded nucleic acids, including hybridized pairs of capture probes or capture/primer probes and target-nucleic acids, that are either a particular number of base pairs shorter or a particular range of base pairs shorter than the full-length of a double-stranded capture-probe or capture/primer probe; and
d) modulating voltages between the annealing-voltage and denaturing-voltage, wherein multiple cycles of modulation are performed on the entire nucleic acid-containing sample while it is inside the fluid chamber, wherein after the final application of the corresponding pH level, the target-nucleic acid is enriched or isolated from the nucleic acid-containing sample by remaining bound to the capture-probes or capture/primer probes on the at least one electrode, wherein the modulating voltages between the annealing-voltage and denaturing-voltage is at a modulation frequency in the range of 0.1-1000 Khz.