US 12,006,515 B2
Composition and method for preventing or treating eye disorder
Yuan-Chieh Lee, Hualien (TW); and Li-Yi Sun, Hualien (TW)
Assigned to Buddhist Tzu Chi Medical Foundation, Hualien (TW)
Filed by Buddhist Tzu Chi Medical Foundation, Hualien (TW)
Filed on Dec. 29, 2020, as Appl. No. 17/136,080.
Claims priority of provisional application 62/954,738, filed on Dec. 30, 2019.
Prior Publication US 2021/0198631 A1, Jul. 1, 2021
Int. Cl. C12N 5/0775 (2010.01); A61P 27/02 (2006.01); C12N 5/00 (2006.01)
CPC C12N 5/0668 (2013.01) [A61P 27/02 (2018.01); C12N 5/0018 (2013.01); C12N 2500/32 (2013.01); C12N 2501/115 (2013.01); C12N 2501/999 (2013.01)] 8 Claims
 
1. A method for preventing or treating environmentally-induced evaporative dry eye syndrome in a subject in need thereof, comprising administering to the eye of said subject a therapeutically effective amount of a formulation comprising a composition comprising a fraction with molecular weights less than 30 kDa prepared by:
obtaining adipose-derived stem cells (ADSCs) from adipose tissue of the subject, wherein the subject is human;
culturing the ADSCs in a medium supplemented with serum and mesenchymal stem cell culture adjuvant (MCA) for 2 to 5 passages,
wherein the medium is selected from the group consisting of alpha minimum essential medium (alpha-MEM), Dulbecco's Modified Eagle's Medium (DMEM), Roswell Park Memorial Institute (RPM!) medium, improved minimum essential medium (IMEM), and Iscove's Modified Dulbecco's Medium (IMDM),
wherein the serum is fetal bovine serum or human serum having a concentration in a range of from 5% to 15% in the medium,
wherein the MCA comprises at least one of fibroblast growth factor 2 (FGF2), N-acetyl-L-cysteine (NAC) and L-ascorbic acid-2-phosphate (AsA2P);
harvesting the medium from the culture of 2 to 5 ADSC passages;
centrifuging the harvested medium followed by filtering; and
obtaining the fraction with molecular weights less than 30 kDa from the filtered medium.