	US 12,006,504 B2
	Production of DHA and other LC PUFAs in plants
Terence A. Walsh, Carmel, IN (US); Daniel Gachotte, Indianapolis, IN (US); Ann Owens Merlo, Carmel, IN (US); Paul G. Roessler, Fort Myers, FL (US); James George Metz, Longmont, CO (US); Scott Bevan, Indianapolis, IN (US); and Jerry M. Kuner, Longmont, CO (US)
Assigned to DSM IP Assets B.V., (NL); and DOW AgroSciences LLC, Indianapolis, IN (US)
Filed by DSM IP Assets B.V., TE Heerlen (NL); and DOW AgroSciences LLC, Indianapolis, IN (US)
Filed on Feb. 11, 2022, as Appl. No. 17/669,945.
Application 16/139,534 is a division of application No. 15/825,107, filed on Nov. 29, 2017, granted, now 10,669,554.
Application 17/669,945 is a continuation of application No. 16/139,534, filed on Sep. 24, 2018, abandoned.
Application 15/825,107 is a continuation of application No. 13/698,412, abandoned, previously published as PCT/US2011/036869, filed on May 17, 2011.
Claims priority of provisional application 61/345,537, filed on May 17, 2010.
Prior Publication US 2022/0170037 A1, Jun. 2, 2022
Int. Cl. C12N 15/82 (2006.01); C12P 7/6427 (2022.01); C12N 9/10 (2006.01); C12N 9/12 (2006.01)

CPC C12N 15/8247 (2013.01) [C12P 7/6427 (2013.01); C12N 9/1029 (2013.01); C12N 9/1288 (2013.01)]

15 Claims

1. A method of obtaining a canola seed oil comprising the step of extracting a canola seed oil from a genetically modified Brassica plant, said genetically modified Brassica plant being genetically modified to express a PUFA synthase system derived from a eukaryotic source, the canola seed oil comprising at least 0.01% docosahexaenoic acid (DHA), wherein said canola seed oil is of the intermediate or side products of gamma-linolenic acid (GLA; 18:3, n-6), stearidonic acid (STA or SDA; 18:4, n-3), dihomo-gamma-linolenic acid (DGLA or HGLA; 20:3, n-6), arachidonic acid (ARA, C20:4, n-6), and eicosatrienoic acid (ETA; 20:3, n-9), so long as such intermediate or side products are not naturally produced by the endogenous FAS system in the wild-type canola plant.