US 12,006,496 B2
Isotachophoresis for purification of nucleic acids
Lewis A. Marshall, Pleasanton, CA (US); Amy L. Hiddessen, Pleasanton, CA (US); Nathan P. Hoverter, Pleasanton, CA (US); Klint A. Rose, Pleasanton, CA (US); and Juan G. Santiago, Pleasanton, CA (US)
Assigned to Purigen Biosystems, Inc., Pleasanton, CA (US)
Filed by Purigen Biosystems, Inc., Pleasanton, CA (US)
Filed on May 1, 2023, as Appl. No. 18/141,641.
Application 18/141,641 is a continuation of application No. 16/997,614, filed on Aug. 19, 2020, granted, now 11,674,132.
Application 16/997,614 is a continuation of application No. 16/046,560, filed on Jul. 26, 2018, granted, now 10,822,603, issued on Nov. 3, 2020.
Application 16/046,560 is a continuation of application No. PCT/US2017/015519, filed on Jan. 28, 2017.
Claims priority of provisional application 62/288,930, filed on Jan. 29, 2016.
Prior Publication US 2023/0357748 A1, Nov. 9, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 27/453 (2006.01); B01L 3/00 (2006.01); C12N 15/10 (2006.01); C12Q 1/6806 (2018.01); G01N 27/447 (2006.01); B81B 1/00 (2006.01); B81B 7/00 (2006.01)
CPC C12N 15/101 (2013.01) [B01L 3/502753 (2013.01); C12N 15/10 (2013.01); C12Q 1/6806 (2013.01); G01N 27/4473 (2013.01); G01N 27/44739 (2013.01); G01N 27/44791 (2013.01); G01N 27/44795 (2013.01); B01L 3/502738 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0867 (2013.01); B81B 1/004 (2013.01); B81B 7/0087 (2013.01); B81B 2201/0278 (2013.01); B81B 2201/05 (2013.01); B81B 2203/0338 (2013.01); G01N 27/44704 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A kit comprising:
(a) microfluidic chip comprising two or more fluidic channels each in fluid communication with a sample reservoir configured to receive a sample, a first buffer reservoir configured to receive a buffer, a second buffer reservoir configured to receive a second buffer, a third buffer reservoir configured to receive a third buffer and an elution reservoir configured to receive an eluate; and
(b) one or more buffers.