US 12,005,454 B2
Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
Rajiv Bharadwaj, Pleasanton, CA (US); Kevin Ness, Pleasanton, CA (US); Debkishore Mitra, Berkeley, CA (US); Donald A. Masquelier, Tracy, CA (US); Anthony Makarewicz, Livermore, CA (US); Christopher Hindson, Pleasanton, CA (US); Benjamin Hindson, Pleasanton, CA (US); and Serge Saxonov, Oakland, CA (US)
Assigned to 10x Genomics, Inc., Pleasanton, CA (US)
Filed by 10X Genomics, Inc., Pleasanton, CA (US)
Filed on Jan. 11, 2021, as Appl. No. 17/145,858.
Application 17/145,858 is a continuation of application No. 16/419,820, filed on May 22, 2019, abandoned.
Application 16/419,820 is a continuation of application No. 15/596,754, filed on May 16, 2017, granted, now 10,343,166, issued on Jul. 9, 2019.
Application 15/596,754 is a continuation of application No. 14/682,952, filed on Apr. 9, 2015, granted, now 9,694,361, issued on Jul. 4, 2017.
Claims priority of provisional application 61/977,804, filed on Apr. 10, 2014.
Prior Publication US 2021/0129148 A1, May 6, 2021
Int. Cl. B01L 3/00 (2006.01); B01L 3/02 (2006.01); G01N 35/00 (2006.01); G01N 35/08 (2006.01); G01N 35/10 (2006.01)
CPC B01L 3/502761 (2013.01) [B01L 3/0241 (2013.01); B01L 3/502784 (2013.01); B01L 2200/0673 (2013.01); B01L 2300/0816 (2013.01); B01L 2400/049 (2013.01); B01L 2400/086 (2013.01); G01N 2035/00237 (2013.01); G01N 35/085 (2013.01); G01N 2035/1034 (2013.01)] 14 Claims
 
1. A microfluidic system, comprising:
a first fluid source fluidly connected to a first channel and comprising a first fluid that comprises an aqueous fluid and a plurality of microcapsules;
an agitating component disposed to agitate the first fluid source;
a second fluid source fluidly connected to a second channel and comprising a second fluid that is immiscible with the aqueous fluid;
a droplet generation junction fluidly connected to a third channel; and
a flow control system that comprises a first independently controlled pressure source that causes the first fluid from the first fluid source to flow along the first channel and a second independently controlled pressure source that causes the second fluid from the second fluid source to flow along the second channel, such that the microcapsules and the second fluid meet at the droplet generation junction to generate a plurality of droplets comprising the microcapsules from the plurality of microcapsules, which plurality of droplets flow along the third channel.