US 12,005,452 B2
Nanoscale biochemical sample preparation and analysis
Ryan T. Kelly, West Richland, WA (US); Ying Zhu, Richland, WA (US); and Richard D. Smith, Richland, WA (US)
Assigned to BATTELLE MEMORIAL INSTITUTE, Richland, WA (US)
Filed by Battelle Memorial Institute, Richland, WA (US)
Filed on Aug. 17, 2021, as Appl. No. 17/404,478.
Application 17/404,478 is a division of application No. 15/897,022, filed on Feb. 14, 2018, granted, now 11,123,732.
Application 15/897,022 is a continuation in part of application No. PCT/US2017/060399, filed on Nov. 7, 2017.
Claims priority of provisional application 62/418,544, filed on Nov. 7, 2016.
Prior Publication US 2022/0080420 A1, Mar. 17, 2022
Int. Cl. B01L 3/00 (2006.01); A61B 10/02 (2006.01); B01J 19/00 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); C12Q 1/6881 (2018.01); C40B 60/04 (2006.01); G01N 1/28 (2006.01); G01N 1/30 (2006.01); G01N 1/34 (2006.01); G01N 30/88 (2006.01); G01N 33/483 (2006.01); G01N 33/68 (2006.01); G02B 21/32 (2006.01)
CPC B01L 3/502753 (2013.01) [A61B 10/02 (2013.01); B01J 19/0046 (2013.01); B01L 3/00 (2013.01); B01L 3/50853 (2013.01); B01L 3/5088 (2013.01); C12M 23/58 (2013.01); C12M 41/00 (2013.01); C12Q 1/6881 (2013.01); C40B 60/04 (2013.01); G01N 1/2813 (2013.01); G01N 1/30 (2013.01); G01N 1/34 (2013.01); G01N 30/88 (2013.01); G01N 33/4833 (2013.01); G01N 33/68 (2013.01); G02B 21/32 (2013.01); B01J 2219/00317 (2013.01); B01J 2219/00367 (2013.01); B01J 2219/00619 (2013.01); B01J 2219/00621 (2013.01); B01J 2219/00659 (2013.01); B01J 2219/00702 (2013.01); B01J 2219/00725 (2013.01); B01J 2219/00743 (2013.01); B01L 2200/142 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0822 (2013.01); B01L 2300/0887 (2013.01); G01N 2001/284 (2013.01); G01N 2030/8831 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A platform for biological sample preparation, comprising:
a substrate comprising at least one reactor vessel having one or more hydrophilic surfaces configured for containment of a biological sample, wherein the hydrophilic surface is formed on an upper surface of a pillar and defines the lateral boundary of the least one reactor vessel and the hydrophilic surfaces have a non-zero total surface area less than 25 mm2;
a spacer containing an aperture, wherein the aperture is dimensioned to surround the at least one reactor vessel when the spacer is positioned on the substrate;
a cover positioned on the spacer; and
a membrane interposed between the spacer and the cover and the membrane overlies the spacer, wherein the membrane has a membrane aperture that is dimensioned to surround the at least one reactor vessel when the membrane is positioned on the spacer, wherein the substrate, spacer and cover are each formed from a material that is transparent to optical light.