US 12,005,448 B2
Systems and methods of using anisotropic nanostructures in microfluidic devices for binding and optional release of molecules and cells
Leonardo Scarabelli, Los Angeles, CA (US); Gail Vinnacombe, Los Angeles, CA (US); Liv Heidenreich, Los Angeles, CA (US); Naihao Chiang, Los Angeles, CA (US); Yao Gong, Los Angeles, CA (US); Paul S. Weiss, Los Angeles, CA (US); and Steven J. Jonas, Los Angeles, CA (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
Appl. No. 17/268,955
Filed by THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
PCT Filed Aug. 16, 2019, PCT No. PCT/US2019/046874
§ 371(c)(1), (2) Date Feb. 16, 2021,
PCT Pub. No. WO2020/037238, PCT Pub. Date Feb. 20, 2020.
Claims priority of provisional application 62/719,505, filed on Aug. 17, 2018.
Prior Publication US 2021/0308671 A1, Oct. 7, 2021
Int. Cl. B01L 3/00 (2006.01); C01G 7/00 (2006.01); G01N 21/64 (2006.01); G01N 21/65 (2006.01); G01N 33/543 (2006.01); G01N 33/574 (2006.01); G02B 21/00 (2006.01); G02B 21/16 (2006.01); B82Y 35/00 (2011.01)
CPC B01L 3/50273 (2013.01) [B01L 3/502761 (2013.01); C01G 7/00 (2013.01); G01N 21/6458 (2013.01); G01N 21/658 (2013.01); G01N 33/54346 (2013.01); G01N 33/57492 (2013.01); G02B 21/006 (2013.01); G02B 21/16 (2013.01); B01L 2300/0838 (2013.01); B01L 2300/0896 (2013.01); B01L 2400/0478 (2013.01); B82Y 35/00 (2013.01); C01P 2004/03 (2013.01); C01P 2004/30 (2013.01); C01P 2004/64 (2013.01); G01N 2021/656 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A microfluidic device for the selective capture and release of cells comprising:
one or more microfluidic channels or capillaries having one or more inner surfaces functionalized with a plurality of in-situ grown gold anisotropic nanostars disposed thereon, wherein the gold anisotropic nanostars exhibit plasmonic activity in one or more wavelength ranges and further comprise a capture ligands specific to a specific type of cell:
one or more pumps configured to pump a sample containing the cells through the one or more microfluidic channels or capillaries, wherein at least some of the cells adhere to the capture ligands; and
a light source configured to deliver a focused beam of light onto the one or more inner surfaces functionalized with the plurality of in-situ grown gold anisotropic nanostars, wherein the focused beam of light is configured to produce localized heating on the gold anisotropic nanostars, which is configured to cause conformation changes in the gold anisotropic nanostars to release the at least some of the cells that adhere to the capture ligands.