CPC B01L 3/502707 (2013.01) [B01L 7/52 (2013.01); B01L 2200/02 (2013.01); B01L 2200/12 (2013.01); B01L 2300/041 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/12 (2013.01); B01L 2300/161 (2013.01); B01L 2300/168 (2013.01); B01L 2300/18 (2013.01)] | 13 Claims |
1. A microfluidic device for thermocycling of a reaction mixture, comprising:
an inlet opening;
an outlet opening;
a flow channel connecting said inlet opening and said outlet opening and defining a flow direction from the inlet opening through the flow channel to the outlet opening, wherein the flow channel comprises a first flow channel surface;
a second flow channel surface opposite to the first flow channel surface;
and an array of wells provided in said first flow channel surface for fluidic communication with the inlet opening and the outlet opening, wherein each well of the wells of the array of wells has a well shape in the first flow channel surface in a form of a regular hexagon elongated in the flow direction such that a length of the well from a first corner to a second opposite corner in the flow direction is greater than a width of the well from a first flat to a second parallel flat wherein the width is perpendicular to the flow direction, wherein the first flow channel surface comprises a first hydrophilicity having a surface contact angle in a range of 30° to 50° and at least a part of the second flow channel surface comprises a second hydrophilicity having a surface contact angle in a range of 80° to 90°, such that said first hydrophilicity is greater than said second hydrophilicity.
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