CPC B01L 3/502 (2013.01) [B01L 2200/16 (2013.01); B01L 2300/042 (2013.01); B01L 2300/044 (2013.01); B01L 2300/047 (2013.01); B01L 2300/14 (2013.01); B01L 2400/0475 (2013.01); B01L 2400/06 (2013.01)] | 11 Claims |
1. An apparatus, comprising:
a fluidics cartridge receivable within a cartridge receptacle of a system and adapted to carry a flow cell, the fluidics cartridge comprising:
a first reagent reservoir containing a first reagent and a buffer reagent reservoir containing a buffer reagent;
a valve; and
a body comprising fluidic lines and an inlet port, the inlet port adapted to be coupled to a gas source, the body carrying the first reagent reservoir, the buffer reagent reservoir, and the valve, the fluidic lines fluidly coupling the inlet port, the first reagent reservoir, the buffer reagent reservoir, the valve, and the flow cell, the inlet port upstream of the first reagent reservoir and the buffer reagent reservoir, the valve downstream of the first reagent reservoir and the buffer reagent reservoir, wherein the first reagent reservoir is to be pressurized by the gas source to enable the first reagent to be flowed under positive pressure;
wherein the valve is movable:
to a first position to fluidly connect the first reagent reservoir to the flow cell to flow the first reagent from the first reagent reservoir into the flow cell under positive pressure to perform a biochemical reaction;
to a second position to fluidly connect the gas source to the flow cell to flow gas into the flow cell to expel at least a portion of the first reagent from the biochemical reaction from the flow cell; and
to a third position to fluidly connect the buffer reagent reservoir to the flow cell to flow the buffer reagent into the flow cell.
|