	US 12,005,265 B2
	Compositions and methods to modulate cell activity
Sarah Stanley, Berkeley, CA (US); Jeffrey Friedman, New York, NY (US); Jonathan S. Dordick, Schenectady, NY (US); and Jeremy Sauer, Princeton, NJ (US)
Assigned to THE ROCKEFELLER UNIVERSITY, New York, NY (US); and RENSSELAER POLYTECHNIC INSTITUTE, Troy, NY (US)
Filed by THE ROCKEFELLER UNIVERSITY, New York, NY (US); and RENSSELAER POLYTECHNIC INSTITUTE, Troy, NY (US)
Filed on Aug. 6, 2020, as Appl. No. 16/986,482.
Application 16/986,482 is a continuation of application No. 15/464,748, filed on Mar. 21, 2017, granted, now 10,765,878.
Application 15/464,748 is a continuation of application No. PCT/US2015/051457, filed on Sep. 22, 2015.
Claims priority of provisional application 62/053,602, filed on Sep. 22, 2014.
Prior Publication US 2021/0052909 A1, Feb. 25, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. A61N 2/00 (2006.01); A61K 48/00 (2006.01); A61N 2/06 (2006.01); C07K 14/705 (2006.01); C12N 15/62 (2006.01); C12N 15/86 (2006.01); A61K 9/51 (2006.01); A61K 47/69 (2017.01); A61P 3/08 (2006.01)

CPC A61N 2/002 (2013.01) [A61K 48/00 (2013.01); A61K 48/005 (2013.01); A61K 48/0075 (2013.01); A61N 2/00 (2013.01); A61N 2/004 (2013.01); A61N 2/06 (2013.01); C12N 15/62 (2013.01); C12N 15/86 (2013.01); A61K 9/51 (2013.01); A61K 47/6929 (2017.08); A61P 3/08 (2018.01); C07K 14/705 (2013.01); C07K 2319/00 (2013.01); C12N 2710/10343 (2013.01); G01N 2800/042 (2013.01)]

5 Claims

1. A method of modulating the activity of a population of cells, comprising the steps of:

(a) providing a population of recombinant cells, said cells comprising a genetic construct comprising a nucleotide sequence which encodes an iron-binding protein fused to a first polypeptide and a nucleotide sequence which encodes a TRPV1^Mutantion channel fused to a second polypeptide, wherein the first polypeptide is a binding partner of the second polypeptide, and

(b) exposing the cells to a static magnetic field, thereby activating the ion channel and modulating the activity of the cells.