US 12,005,099 B2
Targeted antimicrobials and related compositions, methods and systems
Paul J. Jackson, Livermore, CA (US); Brian E. Souza, Livermore, CA (US); Feliza A. Bourguet, Livermore, CA (US); and Matthew A. Coleman, Oakland, CA (US)
Assigned to LAWRENCE LIVERMORE NATIONAL SECURITY, LLC, Livermore, CA (US)
Filed by LAWRENCE LIVERMORE NATIONAL SECURITY, LLC, Livermore, CA (US)
Filed on May 21, 2020, as Appl. No. 16/880,492.
Application 16/880,492 is a continuation of application No. 14/457,035, filed on Aug. 11, 2014, granted, now 10,688,163.
Application 14/457,035 is a continuation of application No. 12/852,358, filed on Aug. 6, 2010, granted, now 8,821,860, issued on Sep. 2, 2014.
Claims priority of provisional application 61/232,345, filed on Aug. 7, 2009.
Prior Publication US 2020/0400521 A1, Dec. 24, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 38/50 (2006.01); A61K 45/06 (2006.01); C12P 1/04 (2006.01); C12Q 1/18 (2006.01); G01L 9/00 (2006.01); G01L 9/08 (2006.01); G01L 19/14 (2006.01)
CPC A61K 38/50 (2013.01) [A61K 45/06 (2013.01); C12P 1/04 (2013.01); C12Q 1/18 (2013.01); G01L 9/0052 (2013.01); G01L 9/008 (2013.01); G01L 9/08 (2013.01); G01L 19/141 (2013.01); C12Y 305/01028 (2013.01); G01N 2333/32 (2013.01); G01N 2333/914 (2013.01); H01L 2224/73265 (2013.01)] 18 Claims
 
1. An antimicrobial composition comprising
a therapeutically effective antimicrobial amount of a targeted antimicrobial protein for Bacillus cereus or a microorganism related thereto and a suitable vehicle, the therapeutically effective antimicrobial amount being at least twice the amount produced in a cell of the Bacillus cereus or the microorganism related thereto,
wherein the targeted antimicrobial protein has a catalytic domain and a binding domain, the targeted antimicrobial protein being BCZK2532 protein having SEQ ID NO:3 and/or a related lysin protein,
wherein the related lysin protein has at least 90% identity with the BCZK2532 protein having SEQ ID NO:3 or a portion thereof having a lytic activity, and
wherein the related lysin protein has an arginine in a position corresponding upon alignment to amino acid residue 100 of SEQ ID NO:3.