	US 12,004,547 B2
	Recovery of nitrate reductase activity
Tina Malling Thorsen, Hoersholm (DK); George Nabin Baroi, Hoersholm (DK); Robin Taponen, Hoersholm (DK); Jakob Soeltoft-Jensen, Hoersholm (DK); and Birgitte Yde, Hoersholm (DK)
Assigned to Chr. Hansen A/S, Hoersholm (DK)
Appl. No. 17/051,844
Filed by Chr. Hansen A/S, Hoersholm (DK)
PCT Filed May 3, 2019, PCT No. PCT/EP2019/061422 § 371(c)(1), (2) Date Oct. 30, 2020, PCT Pub. No. WO2019/211458, PCT Pub. Date Nov. 7, 2019.
Claims priority of application No. 18170807 (EP), filed on May 4, 2018; and application No. 18184186 (EP), filed on Jul. 18, 2018.
Prior Publication US 2021/0315239 A1, Oct. 14, 2021
Int. Cl. A23L 5/41 (2016.01); A23L 13/40 (2023.01); C12N 1/20 (2006.01); C12N 9/06 (2006.01); C12R 1/44 (2006.01)

CPC A23L 5/41 (2016.08) [A23L 13/45 (2016.08); C12N 1/20 (2013.01); C12N 9/0044 (2013.01); C12N 2500/34 (2013.01); C12N 2500/35 (2013.01); C12N 2500/38 (2013.01); C12R 2001/44 (2021.05); C12Y 107/99004 (2013.01)]

18 Claims

1. A method for preserving or increasing nitrate reductase activity of lactic acid bacteria or Micrococcaceae bacteria having nitrate reductase activity, comprising preparing a mixture comprising citric acid or a salt thereof and bacteria having nitrate reductase activity to obtain a mixture, wherein the bacteria having nitrate reductase activity are selected from lactic acid bacteria and Micrococcaceae bacteria, and wherein the bacteria in the mixture are protected from loss of nitrate reductase activity due to one or more of freezing, drying, and freeze-drying, as compared to bacteria formulated without the citric acid or salt thereof.