US 12,326,456 B2
Method of diagnosis of the aesthetic qualities of the skin
Sandra Kanani, Aulnay-sous-Bois (FR); Virginie Piffaut, Aulnay-sous-Bois (FR); Aude Foucher, Aulnay-sous-Bois (FR); and Mark Donovan, Aulnay-sous-Bois (FR)
Assigned to L'OREAL, Paris (FR)
Appl. No. 16/468,073
Filed by L'OREAL, Paris (FR)
PCT Filed Dec. 22, 2017, PCT No. PCT/EP2017/084547
§ 371(c)(1), (2) Date Jun. 10, 2019,
PCT Pub. No. WO2018/115517, PCT Pub. Date Jun. 28, 2018.
Claims priority of application No. 16 63364 (FR), filed on Dec. 23, 2016.
Prior Publication US 2020/0072851 A1, Mar. 5, 2020
Int. Cl. G01N 33/68 (2006.01); C12Q 1/6883 (2018.01); G01N 33/50 (2006.01)
CPC G01N 33/6881 (2013.01) [C12Q 1/6883 (2013.01); G01N 33/5044 (2013.01); C12Q 2600/148 (2013.01); C12Q 2600/158 (2013.01); G01N 2500/04 (2013.01); G01N 2500/10 (2013.01); G01N 2800/20 (2013.01)] 2 Claims
 
1. A method for cosmetic treatment of a skin for which the esthetic qualities are degraded in a subject, wherein the degraded esthetic qualities are selected from the reduction in shine, appearance of a rough texture, or an increase in skin flaccidity in a subject, wherein the subject does not suffer from dermatological lesions or a dermatological pathology, said method comprising the following steps:
a) measuring the level of expression of the gene encoding FLG2 in a skin sample from said subject; wherein the level of expression of the gene encoding FLG2 is determined by measuring the level of expression of the protein encoded by the gene;
b1) determining that the level of expression of the gene encoding FLG2 measured in step a) is statistically lower than a reference value, wherein the reference value is about 166 ng/ml of FLG2; and
c1) treating the skin of the subject with a statistically lower expression level than the reference value with a cosmetic composition suitable for treating rough texture of the skin of the subject; or
b2) determining that the level of expression of the gene encoding FLG2 measured in step a) is statistically higher than a reference value, wherein the reference value is about 140 ng/ml of FLG2 for shine and wherein the reference value is about 82 ng/ml of FLG2 for the flaccidity; and
c2) treating the skin of the subject with a statistically higher expression level than the reference value with a cosmetic composition suitable for treating a reduction in the shine and/or an increase in the flaccidity of the skin of the subject.